Synlett 2016; 27(01): 121-125
DOI: 10.1055/s-0035-1560381
letter
© Georg Thieme Verlag Stuttgart · New York

Synthesis of a Tethered myo-Inositol (1,3,4,5,6)Pentakisphosphate (IP5) Derivative as a Probe for Biological Studies

Mark Gregory*
a   School of Chemistry, Bio21 Institute, The University of Melbourne, 30 Flemington Road, Parkville, Victoria 3052, Australia   Email: m.gregory4@pgrad.unimelb.edu.au   Email: aholmes@unimelb.edu.au
,
Bruno Catimel
b   Walter and Eliza Hall Institute of Medical Research, 1G Royal Parade, Parkville, Victoria 3052, Australia
,
Meng-Xin Yin
a   School of Chemistry, Bio21 Institute, The University of Melbourne, 30 Flemington Road, Parkville, Victoria 3052, Australia   Email: m.gregory4@pgrad.unimelb.edu.au   Email: aholmes@unimelb.edu.au
,
Melanie Condron
b   Walter and Eliza Hall Institute of Medical Research, 1G Royal Parade, Parkville, Victoria 3052, Australia
,
Antony W. Burgess
b   Walter and Eliza Hall Institute of Medical Research, 1G Royal Parade, Parkville, Victoria 3052, Australia
,
Andrew B. Holmes*
a   School of Chemistry, Bio21 Institute, The University of Melbourne, 30 Flemington Road, Parkville, Victoria 3052, Australia   Email: m.gregory4@pgrad.unimelb.edu.au   Email: aholmes@unimelb.edu.au
› Author Affiliations
Further Information

Publication History

Received: 24 September 2015

Accepted after revision: 13 November 2015

Publication Date:
30 November 2015 (online)


This paper is dedicated to Prof. Steven Ley on the occasion of his 70th birthday and in recognition of his pioneering synthesis of the GPI anchor.

Abstract

There is sufficient evidence to suggest that myo-inositol pentakisphosphate is a vital intermediate species in higher inositol phosphate metabolism, however, its biological roles and physiological function in cells remain uncertain. A tethered myo-inositol pentakisphosphate (IP5) derivative with a terminal amine group is synthesised allowing facilitated immobilisation onto M-270 magnetic Dynabeads for pull-down experiments and biosensor chip preparation for surface plasmon resonance studies. The probes are validated by both pull-down and surface plasmon resonance (SPR) studies of the known binding protein GRP-1 (general receptor for phosphoinositides 1), and furthermore by SPR studies of protein kinase B (PKB or AKT) binding.

Supporting Information