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Synfacts 2009(9): 1045-1045
DOI: 10.1055/s-0029-1217660
DOI: 10.1055/s-0029-1217660
Polymer-Supported Synthesis
© Georg Thieme Verlag
Stuttgart ˙ New York
Optical Resolution with Immobilized Enzymes on Nanoparticles
Contributor(s):Yasuhiro Uozumi, Yoshikazu YamamotoK. S. Lee, M. H. Woo, H. S. Kim, E. Y. Lee*, I. S. Lee*
Kyung Hee University, Gyeonggi-do and Kyungsung University, Busan, Republic of Korea
Synthesis of Hybrid Fe3O4-Silica-NiO Superstructures and Their Application as Magnetically Separable High-Performance Biocatalysts
Chem. Commun. 2009, 3780-3782
Kyung Hee University, Gyeonggi-do and Kyungsung University, Busan, Republic of Korea
Synthesis of Hybrid Fe3O4-Silica-NiO Superstructures and Their Application as Magnetically Separable High-Performance Biocatalysts
Chem. Commun. 2009, 3780-3782
Further Information
Publication History
Publication Date:
21 August 2009 (online)
Key words
immobilized enzymes - hybrid nanoparticles - kinetic resolution
Significance
Enzyme-anchored hybrid nanoparticles (HIS-HNP) were prepared by reaction of His-tagged enzymes (epoxide hydrases) with the PEG-HNP-bearing NiO particles (eq. 1). The kinetic resolution of racemic styrene oxide was performed with HIS-HNP to give enantioenriched (S)-styrene oxide (>98% ee). The catalyst was magnetically separated from the reaction mixture and reused five times (eq. 2).
Comment
HNP and PEG-HNP were characterized by ICP-AES, TEM, and SEM analyses. Though the amount of detached enzyme from HIS-HNP was less than 5% during the reaction, the enzymatic activity decreased as the recycling process was repeated.