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Horm Metab Res 2008; 40(8): 528-532
DOI: 10.1055/s-2008-1073162
Original Basic

© Georg Thieme Verlag KG Stuttgart · New York

A High-fructose Diet Impairs Akt and PKCζ Phosphorylation and GLUT4 Translocation in Rat Skeletal Muscle

P. Li 1 , T. Koike 1 , 2 , B. Qin 1 , M. Kubota 1 , Y. Kawata 1 , Y. J. Jia 1 , Y. Oshida 1 , 2
  • 1Department of Sports Medicine, Graduate School of Medicine, Nagoya University, Nagoya, Japan
  • 2Research Center of Health, Physical Fitness and Sports, Nagoya University, Nagoya, Japan
Further Information

Publication History

received 16.05.2007

accepted 15.01.2008

Publication Date:
21 May 2008 (online)

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Abstract

The molecular mechanism of insulin resistance induced by high-fructose feeding is not fully understood. The present study investigated the role of downstream signaling molecules of phosphatidylinositol 3-kinase (PI3K) in the insulin-stimulated skeletal muscle of high-fructose-fed rats. Rats were divided into chow-fed and fructose-fed groups. The results of the euglycemic clamp study (insulin infusion rates: 6 mU/kg BW/min) showed a significant decrease in the glucose infusion rate (GIR) and the metabolic clearance rate of glucose (MCR) in fructose-fed rats compared with chow-fed rats. In skeletal muscle removed immediately after the clamp procedure, high-fructose feeding did not alter protein levels of protein kinase B (PKB/Akt), protein kinase C ζ (PKCζ), or glucose transporter 4 (GLUT4). However, insulin-stimulated phosphorylation of Akt and PKCζ and GLUT4 translocation to the plasma membrane were reduced. Our findings suggest that insulin resistance in fructose-fed rats is associated with impaired Akt and PKCζ activation and GLUT4 translocation in skeletal muscle.

Key words

insulin resistance - euglycemic clamp - protein kinase - glucose transporter - insulin signaling

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Correspondence

Y. OshidaMD, PhD 

Department of Sports Medicine

Graduate School of Medicine

Nagoya University

464-8601 Nagoya

Japan

Phone: +81/52/789 39 61

Fax: +81/52/789 39 57

Email: oshida@htc.nagoya-u.ac.jp