Subscribe to RSS
DOI: 10.1055/s-2007-978649
© Georg Thieme Verlag Stuttgart · New York
Nitric Oxide Decreases Insulin Resistance Induced by High-Fructose Feeding
Publication History
2000
2000
Publication Date:
19 April 2007 (online)
![](https://www.thieme-connect.de/media/hmr/200009/lookinside/thumbnails/10.1055-s-2007-978649-1.jpg)
The effect of nitric oxide (NO) on insulin resistance was studied in high-fructose-fed rats. A sequential hyperinsulinemic euglycemic clamp procedure was employed (insulin infusion rates: 3 and 30 mU/kg BW/min) in 12 high-fructose-fed rats and 12 chow-fed rats while awake. Half of the high-fructose-fed and the chow-fed rats, respectively, were continuously given sodium nitroprusside (SNP, 3 ng/kg BW/min) during the clamp study. Blood glucose was clamped at the fasting level in each rat. Plasma insulin levels during the 3 and 30 mU/kg BW/min insulin infusions were 30 and 400 µU/ml, respectively. Metabolic clearance rate of glucose (MCR) was regarded as an index of whole body insulin action. At both 3 and 30 mU/kg BW/min insulin infusions, high-fructose feeding showed a significant decrease in MCR compared with the chow-fed rats. However, decreased MCRs were stimulated by SNP administration and reached similar levels as the chow-fed rats. SNP infusion did not influence MCRs in the chow-fed rats. Therefore it could be concluded that NO can improve insulin resistance induced by high-fructose feeding.
Key words
Euglycemic Clamp Technique - Insulin Action - Sodium Nitroprusside