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Thromb Haemost 2004; 91(03): 587-594
DOI: 10.1160/TH03-10-0644
Wound Healing and Inflammation/Infection
Schattauer GmbH

Moderate-intensity exercise suppresses platelet activation and polymorphonuclear leukocyte interaction with surface-adherent platelets under shear flow in men

Jong-Shyan Wang
1   Graduate Institute of Rehabilitation Science and Center for Gerontological Research, Chang Gung University, Taoyuan, Taiwan
,
Chang-Hui Liao
2   Graduate Institute of Natural Products, Chang Gung University, Taoyuan, Taiwan
› Author AffiliationsFinancial support The study was support under the National Science Council Grant NSC 92-2314-B-182-021.
Further Information

Publication History

Received 23 October 2003

Accepted after revision 04 January 2003

Publication Date:
05 December 2017 (online)

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Summary

The reciprocal modulation of platelet and polymorphonuclear leukocyte (PMN) activities is important in the pathogenesis of thrombosis and inflammation. This study investigated how moderate exercise affects shear-induced platelet activation and subsequent PMNs interaction with platelet-related thrombi under shear flow. Sixteen sedentary healthy men engaged in moderate exercise (about 60% V·O2max) on a bicycle ergometer. Platelet activation, PMNs interaction with surface-adherent platelets, and PMN-dependent inhibition of platelet activation under shear flow were measured both before and immediately after exercise. The results of this study can be summarized as follows: (1) moderate exercise was associated with lower extents of shear-induced platelet adhesion and aggregation, binding of von Willbrand factor (vWF) to platelets, and glycoprotein IIb/IIIa activation and P-selectin expression on platelet than at rest; (2) the velocity and percentage of rolling PMNs increased while the number of PMNs remaining bound to surface-adherent platelets decreased after moderate exercise; (3) although treating the PMNs with oxidized-low density lipoprotein (Ox-LDL) enhanced PMNs interaction with surface-adherent platelets, moderate exercise suppressed the enhancement of platelet-PMN interaction by Ox-LDL; (4) moderate exercise decreased platelet [Ca2+]i elevation induced by ADP and platelet [Ca2+]i levels mediated by PMN and Ox-LDL-treated PMN; and (5) plasma and PMN-derived nitric oxide metabolites and plasma vWF antigen and activity increased after moderate exercise, whereas plasma and platelet-derived soluble P-selectin levels remained unchanged in response to exercise. Therefore, we conclude that moderate-intensity exercise suppresses shear-induced platelet activation and subsequent PMNs adhesion to platelets deposited at sites of vascular injury under flow, thereby reducing the risks of vascular thrombosis and inflammation.

Keywords

Moderate exercise - shear stress - platelet-PMN interaction
 

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