Planta Med 2007; 73(10): 1127-1132
DOI: 10.1055/s-2007-981560
Analytical Methods
Original Paper
© Georg Thieme Verlag KG Stuttgart · New York

Preparation of a Novel Monoclonal Antibody against the Antimalarial Drugs, Artemisinin and Artesunate

Hiroyuki Tanaka1 , Waraporn Putalun2 , Wanchai De-Eknamkul3 , Oraphan Matangkasombut4 , Yukihiro Shoyama1
  • 1Department of Pharmacognosy, Graduate School of Pharmaceutical Sciences, Kyushu University, Fukuoka, Japan
  • 2Faculty of Pharmaceutical Sciences, Khon Kaen University, Khon Kaen, Thailand
  • 3Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok, Thailand
  • 4Faculty of Pharmacy, Rangsit University, Pathumthani, Thailand
Further Information

Publication History

Received: March 2, 2007 Revised: May 30, 2007

Accepted: June 4, 2007

Publication Date:
05 July 2007 (online)

Abstract

A monoclonal antibody (MAb) 1C1 against artemisinin was prepared by a cell fusion with splenocytes and aminopterin-sensitive myeloma cells, SP2/0. An artesunate-BSA conjugate was used as immunogen to raise antibodies specific to artemisinin. The prepared anti-artemisinin MAb-1C1 have a novel characteristic which shows a specificity to compounds that are structurally related to artemisinin such as artesunate (630 %) and dihydroartemisinin (29.9 %). By using MAb-1C1, a specific and reliable ELISA was developed for the detection of compounds structurally related to artemisinin. The system shows a full measuring range from 2 to 20 μg/mL in the case of artemisinin and from 4 to 125 ng/mL in the case of artesunate in the competitive ELISA, and was validated to be of use for surveying and breeding of Artemisia annua containing a high amount of artemisinin as well as for the characterization of the pharmacokinetics of artemisinin and its derivatives.

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Hiroyuki Tanaka

Department of Pharmacognosy

Graduate School of Pharmaceutical Sciences

Kyushu University

3-1-1 Maidashi

Higashi-ku

Fukuoka 812-8582

Japan

Email: htanaka@phar.kyushu-u.ac.jp