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Horm Metab Res 1989; 21(1): 8-10
DOI: 10.1055/s-2007-1009137
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© Georg Thieme Verlag, Stuttgart · New York

Effects of Acetylcholine on Thyrotropin-Releasing Hormone Release from the Rat Caecum in Vitro

T. Mitsuma1 , Y. Hirooka1 , K. Nakata2 , T. Nogimori2
  • 1Fourth Department of Internal Medicine, Aichi Medical University, Nagakute, Aichi, Japan
  • 2Department of Internal Medicine, Konanshowa Hospital, Kochino, Aichi, Japan
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Publikationsverlauf

1988

1988

Publikationsdatum:
14. März 2008 (online)

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Summary

Effects of acetylcholine on the release of thyrotropin-releasing hormone (TRH) from the rat caecum in vitro were studied. The rat caecum was incubated in medium 199 with 1.0 mg/ml of bacitracin and 100 μg/ml of ascorbic acid (pH 7.4) (medium). The amount of TRH release into the medium was measured by radioimmunoassay.

The immunoreactive TRH (ir-TRH) release from the rat caecum was enhanced significantly in a dose-related manner with the addition of acetylcholine, but not changed with atropine. The stimulatory effect of acetylcholine on ir-TRH release from the rat caecum was blocked with an addition of atropine. Elution profile of acid-methanol-extracted rat caecum on Sephadex G-10 was identical to that of synthetic TRH.

The findings suggest that the cholinergic system stimulates TRH release from the rat caecum in vitro.

Key-Words

Thyrotropin-Releasing Hormone - Acetylcholine - Atropine - Caecum - Rat

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