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Horm Metab Res 1994; 26(1): 43-45
DOI: 10.1055/s-2007-1000770
Originals Clinical

© Georg Thieme Verlag, Stuttgart · New York

Enzyme-Linked Immunosorbent Assay (ELISA) Method for Screening of Non-Classical Steroid 21-Hydroxylase Deficiency

Hajime Ueshiba, Michele Zerah, Maria I. New
  • The New York Hospital-Cornell Medical Center, New York, U.S.A.
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Publication History

1993

1993

Publication Date:
14 March 2008 (online)

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Summary

Morning salivary 17-hydroxyprogesterone (17-OHP) determination is a useful method to diagnose nonclassical 21-hydroxylase deficiency (NC 21-OHD). In ethnic groups with high frequency of NC 21-OHD, mass screening is thought to be necessary. A pilot screening test for NC 21-OHD was performed by measuring morning salivary 17-OHP by enzyme-linked immunosorbent assay (ELISA). Two individuals were found NC 21-OHD from 230 subjects. Results of this ELISA were well correlated with those of established radioimmunoassay. ELISA can be performed in any laboratory because of non-radioactivity and the procedure is very easy by the use of a microplate. This ELISA is suitable for large scale population studies. We conclude that morning salivary 17-OHP determination by ELISA is a useful method for NC 21-OHD screening test.

Key words

Salivary 17-Hydroxyprogesterone (17-OHP) - Non-Classical 21-Hydroxylase Deficiency (NC 21-OHD) - Enzyme-Linked Immunosorbent Assay (ELISA)

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