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Planta Med 1988; 54(6): 504-506
DOI: 10.1055/s-2006-962530
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© Georg Thieme Verlag Stuttgart · New York

Production of the Alkaloids Emetine and Cephaeline in Callus Cultures of Cephaelis ipecacuanha

Sumita Jha1 , N. P. Sahu2 , Shashi B. Mahato2
  • 1Centre of Advanced Study, Department of Botany, University of Calcutta, 35, Ballygunge Circular Road, Calcutta 700019, India.
  • 2Indian Institute of Chemical Biology, 4, Raja S. C. Mullick Road, Jadavpur, Calcutta 700032, India.
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Publication History

1988

Publication Date:
24 January 2007 (online)

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Abstract

Callus cultures were established from hypocotyl explants of Cephaelis ipecacuanha, emetine and cephaeline were identified by HPLC in callus cultures. The effect of macrosalts of different basal medium and hormones on growth and alkaloid accumulation in callus tissues was investigated. Best growth in terms of wet/dry weight was obtained on media containing macrosalts of B5 supplemented with 3% sucrose, 8 mg l-1 IBA, 4 mg l-1 IAA, and 4 mg l-1 NAA. The highest yields of alkaloids were detected in medium containing macrosalts of SH with same hormone combination.