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Planta Med 1988; 54(3): 210-214
DOI: 10.1055/s-2006-962406
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© Georg Thieme Verlag Stuttgart · New York

The Coupling of Catharanthine and Vindoline to Form 3′,4′-Anhydrovinblastine by Haemoproteins and Haemin

Anne Goodbody1 , Tsuyoshi Endo2 , John Vukovic1 , Masanaru Misawa1
  • 1Allelix Inc., 6850 Goreway Drive., Mississauga, Ontario, L4V 1P1, Canada.
  • 2Research Center for Cell and Tissue Culture, Faculty of Agriculture, Kyoto University, Sakyo-ku, Kyoto 606, Japan.
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Publication History

1987

Publication Date:
24 January 2007 (online)

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Abstract

The enzymic coupling of catharanthine and vindoline can be performed with plant peroxidases in a reaction which yields 3′,4′-anhydrovinblastine (AVLB) as the major product. This has led to an investigation of other sources of peroxidase activity and haem. High levels of AVLB were obtained using microperoxidase (haem-undecapeptide) and haemin, and lower yields were also detected with lactoperoxidase, cytochrome C, and haemoglobin. The activity with haemin and microperoxidase has been optimized and it is concluded that, in comparison with horseradish peroxidase, these systems provide an improved means of coupling.