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Planta Med 1999; 65(8): 773-774
DOI: 10.1055/s-2006-960866
DOI: 10.1055/s-2006-960866
Letters
© Georg Thieme Verlag Stuttgart · New York
Characterization of Echinacea Species and Detection of Possible Adulterations by RAPD Analysis
Further Information
Publication History
1999
1999
Publication Date:
04 January 2007 (online)
Abstract
In this report we describe the discrimination of all three pharmaceutically relevant Echinacea species by random amplified polymorphic DNA (RAPD) analysis. Genomic DNA prepared from either fresh root material or dried roots was probed in polymerase chain reactions (PCR) using several arbitrary primers. Distinct banding patterns were obtained for E. purpurea, E. angustifolia var. angustifolia, and E. pallida, respectively. Individual Echinacea drugs could be identified within mixtures containing variable amounts of individual drugs. We propose to use RAPD analysis as a rapid and reliable test for proving the identity of Echinacea root drugs.