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Planta Med 1994; 60(2): 158-162
DOI: 10.1055/s-2006-959441
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© Georg Thieme Verlag Stuttgart · New York

Tropane Alkaloids in Transformed Roots of Datura quercifolia

Jean-Marc Dupraz, Philippe Christen, Ilias Kapetanidis
  • Department of Pharmacognosy, University of Geneva, Sciences II, 30, quai Ernest Ansermet, CH-1211 Geneva 4, Switzerland
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Publication History

1993

1993

Publication Date:
04 January 2007 (online)

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Abstract

Hairy root cultures of Datura quercifolia were established following infection with Agrobacterium rhizogenes strain LBA 9402. Eight tropane alkaloids were identified in the hairy roots, hyoscyamine being the major constituent. The growth and the hyoscyamine content of transformed roots were investigated under various conditions. Gamborg B5, Murashige and Skoog, and Woody Plant media were tested. Gamborg B5 medium was the best for growth as well as for hyoscyamine accumulation. The influence of sucrose concentration was examined and a 5% concentration was found to be the most appropriate for growth and for alkaloid production. After 35 days of incubation in this medium, the hyoscyamine content of the roots was 1.24% based on dry weight. The influence of gibberellic acid and of Amberlite XAD-4 resin on hyoscyamine production was tested.

Key words

Datura quercifolia - Solanaceae - Agrobacterium rhizogenes - tropane alkaloids - GC-MS - HPLC - sucrose - gibberellic acid - Amberlite XAD-4 resin