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Planta Med 1994; 60(2): 146-148
DOI: 10.1055/s-2006-959438
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© Georg Thieme Verlag Stuttgart · New York

Mass Production of Ginseng (Panax ginseng) Embryoids on Media Containing High Concentrations of Sugar1

Isao Asaka2 , Ichio Ii2 , Masao Hirotani3 , Yoshihisa Asada3 , Takafumi Yoshokawa3 , Tsutomu Furuya3
  • 2Life Science Center, Iwaki Glass Co. Ltd., Gyoda 1-50-1, Funabashi-shi, Chiba 273, Japan
  • 3School of Pharmaceutical Sciences, Kitasato University, Minato-ku, Tokyo 108, Japan
1 Part 87 in the series “Studies on Plant Tissue Culture”. For Part 86 see Ref.(1)
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Publication History

1993

1993

Publication Date:
04 January 2007 (online)

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Abstract

A lot of ginseng embryoids were produced by culturing on high concentrations of sugar media from the embryogenic tissues obtained by moderately high temperature treatment. When the sucrose concentration was 100 g/l, the number of embryoids produced were over 100 pieces per g of inoculum weight. It was about ten times of that produced by culturing on 30 g/l of sucrose. Glucose showed an effect similar to sucrose on the basis of weight percentage. However, mannitol did not show this effect. The embryoids obtained by these processes redifferentiated to normal plantlets on culturing on the medium containing 30 g/l of sucrose. The saponin components of the tissue containing embryoids showed a similar pattern to those of natural ginseng by HPLC.

Key words

Panax ginseng - Araliaceae - tissue culture - embryoid - propagation - sugar