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Horm Metab Res 2006; 38(11): 721-726
DOI: 10.1055/s-2006-955093
Original Basic

© Georg Thieme Verlag KG Stuttgart · New York

Fatty Acids Inhibit Intramyocellular Triglyceride Synthesis and Turnover Acutely in High Fat-fed Obese Rats

ZK. Guo 1 , L. Zhou 1
  • 1Endocrine Research Unit, Mayo Foundation, Rochester, USA
Further Information

Publication History

Received 27 March 2006

Accepted after revision 12 June 2006

Publication Date:
16 November 2006 (online)

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Abstract

Obesity is associated with hyperlipidemia and enlarged intramyocellular triglyceride (imcTG) stores. The latter is strongly correlated with muscle insulin resistance. However, whether hyperlipidemia plays a role in imcTG accumulation is unknown. In the present study, the effects of plasma fatty acids on imcTG fractional turnover rate (FTR) and synthesis in skeletal muscle of high fat-fed obese rats have been examined using pulse-chase technique. imcTG was prelabeled (pulse) by continuous infusion of U-14Cglycerol and then the loss of 14C-labels from imcTG was chased while exogenous fatty acids were infused at 0 (saline), 1 (L) or 3 (H) μmol/kg/min. imcTG synthesis was determined using 2-3Hglycerol during the chase. L and H fatty acid infusions raised plasma fatty acids by 14% (p=0.02) and 30% (p=0.001), respectively, while plasma insulin and glycerol and the rate of glycerol appearance remained unchanged (p>0.05). imcTG FTR was suppressed by 36-40% and 48% in gastrocnemius and tibialis anterior, respectively (both p<0.05), and imcTG synthesis was suppressed by 50-60% in the same muscles (both p<0.05). In contrast, neither turnover nor synthesis of imcTG in soleus was affected by fatty acid infusion (p>0.05). imcTG content and the activities of diglyceride acyltransferase and hormone sensitive lipase were not affected by fatty acid infusion. The findings suggested that elevated plasma fatty acids suppress imcTG turnover and synthesis simultaneously and thus do not appear to promote imcTG accumulation in this obesity model at least in short term.

Key words

Skeletal muscle - free fatty acids - triglycerides - turnover - synthesis - obesity

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Correspondence

ZengKui Guo

5-194 Joseph·Mayo Foundation

Rochester

MN 55905

USA

Phone: +1/507/255 64 61

Fax: +1/507/255 48 28

Email: guo.zengkui@mayo.edu

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