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Planta Med 2006; 72(9): 786-791
DOI: 10.1055/s-2006-931607
Original Paper
Pharmacology
© Georg Thieme Verlag KG Stuttgart · New York

Deoxypodophyllotoxin (DPT) Inhibits Eosinophil Recruitment into the Airway and Th2 Cytokine Expression in an OVA-Induced Lung Inflammation

Chang Xin Lin1 , Eunkyung Lee1 , Mei Hua Jin1 , Jumin Yook1 , Zhejiu Quan1 , Kyungmi Ha1 , Tae Chul Moon1 , Mi Jin Kim2 , Keuk Jun Kim2 , Seung Ho Lee1 , Hyeun Wook Chang1
  • 1College of Pharmacy, Yeungnam University, Gyongsan, Korea
  • 2Department of Pathology, College of Medicine, Yeungnam University, Daegu, Korea
Further Information

Publication History

Received: December 20, 2005

Accepted: April 4, 2006

Publication Date:
29 May 2006 (online)

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Abstract

The effect of deoxypodophyllotoxin (DPT) isolated from Anthriscus sylvestris Hoffm. was evaluated in an in vivo animal model for antiasthmatic activity. DPT (1.0 to 5 mg/kg) was given orally to ovalbumin (OVA)/alum-induced asthmatic mice. DPT reduced the number of infiltrated eosinophils in bronchoalveolar lavage (BAL) fluid in a dose-dependent manner. Dexamethasone (5 mg/kg), which was used as a positive control, also strongly inhibited the number of infiltrated eosinophils. The effect of DPT on a transcript profile in a murine asthma model was determined by RT-PCR, which showed that DPT decreased the mRNA levels of the Th2 cytokines. Northern blot analysis showed that DPT also reduced both the eotaxin and arginase I mRNA levels in a dose-dependent manner.

Key words

Deoxypodophyllotoxin (DPT) - eosinophils - bronchoalveolar lavage (BAL) - asthma - Th2 cytokines

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Prof. Hyeun Wook Chang

College of Pharmacy

Yeungnam University

Gyongsan 712-749

Korea

Phone: +82-53-810-2811

Fax: +82-53-810-4654

Email: hwchang@yumail.ac.kr

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