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DOI: 10.1055/s-2006-923883
Georg Thieme Verlag Stuttgart KG · New York
Auxin Biosynthesis in Maize
Publication History
Received: September 29, 2005
Accepted: January 11, 2006
Publication Date:
19 April 2006 (online)
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Abstract
For the biosynthesis of the phytohormone indole-3-acetic acid (IAA), a number of tryptophan-dependent and ‐independent pathways have been discussed. Maize is an appropriate model system to analyze IAA biosynthesis particularly because high quantities of IAA conjugates are stored in the endosperm. This allowed precursor feeding experiments in a kernel culture system followed by retrobiosynthetic NMR analysis, which strongly suggested that tryptophan-dependent IAA synthesis is the predominant route for auxin biosynthesis in the maize kernel. Two nitrilases ZmNIT1 and ZmNIT2 are expressed in seeds. ZmNIT2 efficiently hydrolyzes indole-3-acetonitrile (IAN) to IAA and thus could be involved in auxin biosynthesis. Redundant pathways, e.g., via indole-3-acetaldehyde could imply that multiple mutants will be necessary to obtain IAA-deficient plants and to conclusively identify relevant genes for IAA biosynthesis.
Key words
Auxin - indole-3-acetic acid - tryptophan - maize - nitrilase.
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E. Glawischnig
Lehrstuhl für Genetik
Technische Universität München
Am Hochanger 8
85350 Freising
Germany
Email: egl@wzw.tum.de
Guest Editor: R. Reski