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Horm Metab Res 2001; 33(3): 181-185
DOI: 10.1055/s-2001-14934
Original Clinical
© Georg Thieme Verlag Stuttgart · New York

Cardiac Natriuretic Peptides and Pancreatic Islet Blood Flow in Anesthetized Rats

P-O. Carlsson, A. Andersson, L. Jansson
  • Department of Medical Cell Biology, Uppsala University, Uppsala, Sweden
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Publication History

Publication Date:
31 December 2001 (online)

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The aim of the study was to evaluate effects of cardiac natriuretic peptides on splanchnic circulation, especially to the pancreatic islets. Pentobarbital-anesthetized rats were infused intravenously (0.01 ml/min for 20 min] with saline, atrial natriuretic peptide (ANP; 0.25 or 0.5 µg/kg BW/min), brain natriuretic peptide (BNP; 0.5 µg/kg BW/min) or C-type natriuretic peptide (CNP; 0.5 or 2.0 µg/kg BW/min). Splanchnic blood perfusion was then measured with a microsphere technique. Mean arterial blood pressure was decreased by ANP and BNP, but not by CNP. The animals given the highest dose of ANP became markedly hypoglycemic, whilst no such effects were seen in any of the other groups of animals. Total pancreatic blood flow was decreased by the highest dose of CNP, whereas no change was seen after administration of the other peptides. Islet blood flow was increased by the highest dose of ANP. Neither BNP nor CNP affected islet blood flow. None of the natriuretic peptides influenced duodenal, colonic or arterial hepatic blood flow. It is concluded that cardiac natriuretic peptides exert only minor effects on splanchnic blood perfusion in anesthetized rats. However, islet blood perfusion may be influenced by ANP.

Key words:

Splanchnic Organs - Atrial Natriuretic Peptide - Brain Natriuretic Peptide - C-type Natriuretic Peptide - Hypoglycemia

References

  • 1 De Bold A J, Borenstein H B, Veress A T, Sonnenberg H. A rapid and important natriuretic response to intravenous injection of atrial myocardial extracts in rats.  Life Sci. 1981;  28 89-94
    CrossrefPubMedGoogle Scholar
  • 2 Clerico A, Iervasi G, Mariani G. Pathophysiologic relevance of measuring the plasma levels of cardiac natriuretic peptide hormone in humans.  Horm Metab Res. 1999;  31 487-498
    PubMedGoogle Scholar
  • 3 Sudoh T, Minamino N, Kanagawa K, Matsuo H. C-type natriuretic peptide (CNP). A new member of the natriuretic peptide family identified in porcine brain.  Biochem Biophys Res Commun. 1990;  168 863-870
    CrossrefPubMedGoogle Scholar
  • 4 Kullo I J, Burnett JC J r. C-type natriuretic peptide. In: Sowers JR (ed.). Contemporary Endocrinology: endocrinology of the vasculature. Humana Press Inc Totowa, NJ; 1996: 79-93
    Google Scholar
  • 5 Schulz-Knappe P, Forssmann K, Herbst F, Hock D, Pipkorn R, Forssmann W G. Isolation and structural analysis of a new peptide of cardiodilatin-(ANP)-family, extracted from human urine.  Klin Wschr. 1988;  66 752-759
    CrossrefPubMedGoogle Scholar
  • 6 Nicholls M G. Minisymposium: the natriuretic peptide hormones. The natriuretic peptides in heart failure.  J Intern Med. 1995;  235 515-526
    PubMedGoogle Scholar
  • 7 Richards M A, Nicholls M G, Espiner E A. Natriuretic peptides.  Clin Sci. 1995;  88 18-21
    PubMedGoogle Scholar
  • 8 Yoshibayashi M, Saito Y, Nakao K. Brain natriuretic peptide versus atrial natriuretic peptide - physiological and pathophysiological significance in children and adults: a review.  Eur J Endocrinol. 1996;  135 265-268
    PubMedGoogle Scholar
  • 9 Schrier R W, Abraham W T. Hormones and hemodynamics in heart failure.  New Engl J Med. 1999;  341 577-585
    CrossrefPubMedGoogle Scholar
  • 10 Caramelo C, Fernandez-Cruz A, Villamediana L M, Sanz E, Rodriguez-Puyol D, Hernando L, Lopez-Novoa J M. Systemic and regional haemodynamic effects of a synthetic atrial natriuretic peptide in conscious rats.  Clin Sci. 1986;  71 323-325
    PubMedGoogle Scholar
  • 11 Criscione L, Burdet R, Hänni H, Kamber B, Truog A, Hofbauer K G. Systemic and regional hemodynamic effects of atriopeptin II in anesthetized rats.  J Cardiovasc Pharmacol. 1987;  9 135-141
    PubMedGoogle Scholar
  • 12 Shen Y -T, Young M A, Ohanian J, Graham R M, Vatner S F. Atrial natriuretic factor-induced systemic vasoconstriction in conscious dogs, rats and monkeys.  Circ Res. 1990;  66 647-661
    PubMedGoogle Scholar
  • 13 Woods R L, Jones M JM. Atrial, B-type, and C-type natriuretic peptides cause mesenteric vasoconstriction in conscious dogs.  Am J Physiol. 1999;  276 R1443-R1452
    PubMedGoogle Scholar
  • 14 Woods R L, Smolich J J. Regional blood flow effects of ANP in conscious dogs: preferential gastrointestinal vasoconstriction.  Am J Physiol. 1991;  261 H1961-H1969
    PubMedGoogle Scholar
  • 15 Ühlinger E, Weidmann P, Gnaedinger M P, Hasler L, Bachmann C, Shaw S, Hellmüller B, Lang R E. Increase in circulating insulin induced by atrial natriuretic peptide (ANP) in normal humans.  J Cardiovasc Pharmacol. 1986;  8 1122-1129
    PubMedGoogle Scholar
  • 16 Fehmann H C, Noll B, Göke B, Trautmann M E, Arnold R. Atrial natriuretic factor has a weak insulinotropic action in the isolated perfused rat pancreas.  Res Exp Med. 1990;  190 253-258
    PubMedGoogle Scholar
  • 17 Lee B, Laychock S G. Atrial natriuretic peptide and cyclic nucleotides affect glucose-induced Ca2 + responses in single pancreatic islet beta-cells: correlation with (Ca2 +-Mg2 +)-ATPase activity.  Diabetes. 1997;  46 1312-1318
    PubMedGoogle Scholar
  • 18 Chabot J G, Morel G, Kopelman H, Belles-Isles M, Heisler S. Atrial natriuretic factor and exocrine pancreas: autoradiographic localization of binding sites and ultrastructural evidence for internalisation of endogenous ANF.  Pancreas. 1987;  2 404-413
    PubMedGoogle Scholar
  • 19 Adeghate E, Ember Z, Donath T, Pallot D J, Singh J. Immunohistochemical identification and effects of atrial natriuretic peptide, pancreastatin, leucine-enkephalin, and galanin in the porcine pancreas.  Peptides. 1996;  17 503-509
    CrossrefPubMedGoogle Scholar
  • 20 Verspohl E J, Ammon H PT. Atrial natriuretic peptide (ANP) acts via specific binding sites on cGMP system of rat pancreatic islets without affecting insulin release.  Naunyn-Schmiedeberg’s Arch Pharmacol. 1989;  339 348-353
    PubMedGoogle Scholar
  • 21 Verspohl E J, Kuhn M, Ammon H PT. RIN m5F (rat insulinoma) cells possess receptors for atrial natriuretic peptide (ANP) and a functioning cGMP system.  Horm Metab Res. 1988;  20 700-701
    PubMedGoogle Scholar
  • 22 Straub R H, Hall C, Krämer B K, Elbracht R, Palitzsch K -D, Lang B, Schölmerich J. Atrial natriuretic factor and digoxin-like immunoreactive factor in diabetic patients: their interrelation and the influence of the autonomic nervous system.  J Clin Endocrinol Metab. 1996;  81 3385-3389
    CrossrefPubMedGoogle Scholar
  • 23 Jansson L, Hellerström C. Stimulation by glucose of the blood flow to the pancreatic islets of the rat.  Diabetologia. 1983;  25 45-50
    PubMedGoogle Scholar
  • 24 Jansson L, Hellerström C. A rapid method of visualizing the pancreatic islets for studies of islet capillary blood flow using non-radioactive microspheres.  Acta Physiol Scand. 1981;  113 371-374
    PubMedGoogle Scholar
  • 25 Jansson L. The regulation of pancreatic islet blood flow.  Diab Metab Rev. 1994;  10 407-414
    PubMedGoogle Scholar
  • 26 Amin J, Carretero O A, Ito S. Mechanisms of action of atrial natriuretic factor and C-type natriuretic peptide.  Hypertension. 1996;  27 684-687
    PubMedGoogle Scholar
  • 27 Svensson A M, Östenson C -G, Sandler S, Efendic S, Jansson L. Inhibition of nitric oxide synthase by NG-nitro-L-arginine causes a preferential decrease in pancreatic islet blood flow in normal rats and spontaneously diabetic GK rats.  Endocrinology. 1994;  135 849-853
    CrossrefPubMedGoogle Scholar
  • 28 Vetterlein F, Senske D, Bornkessel C, Schmidt G. Effects of tolbutamide on blood flow in islets and exocrine tissue of the rat pancreas.  Eur J Pharmacol. 1985;  113 395-398
    CrossrefPubMedGoogle Scholar
  • 29 Sparrow R A, Beckingham I J. Islet blood flow following insulin administration.  J Anat. 1989;  163 75-81
    PubMedGoogle Scholar
  • 30 Meyer H H, Vetterlein F, Schmidt G, Hasselblatt A. Measurement of blood flow in pancreatic islets of the rat: effect of isoproterenol and norepinephrine.  Am J Physiol. 1982;  242 E298-E304
    PubMedGoogle Scholar
  • 31 Jansson L. Influence of adrenaline on blood perfusion and vascular conductance of the whole pancreas and the islets of Langerhans in the rat.  Arch Int Pharmacodyn Ther. 1991;  313 90-97
    PubMedGoogle Scholar
  • 32 Woods R L. Vasoconstrictor actions of atrial natriuretic peptide in the splanchnic circulation of anesthetized dogs.  Am J Physiol. 1998;  275 R1822-R1832
    PubMedGoogle Scholar
  • 33 Lappe R W, Todt J A, Wendt R L. Hemodynamic effects of infusion versus bolus administration of atrial natriuretic factor.  Hypertension. 1986;  10 866-873
    PubMedGoogle Scholar
  • 34 Barbee R W, Perry B D, Ré R N, Murgo J P, Field L J. Hemodynamics in transgenic mice with overexpression of atrial natriuretic factor.  Circ Res. 1994;  74 747-751
    PubMedGoogle Scholar

L. Jansson

Department of Medical Cell Biology
Biomedical Centre

Box 571
SE-751 23 Uppsala
Sweden


Email: E-mail:Leif.Jansson@medcellbiol.uu.se

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