Planta Med 2000; 66(8): 762-764
DOI: 10.1055/s-2000-9567
Letter
© Georg Thieme Verlag Stuttgart · New York

Protective Effects of Green Tea Catechins against Asbestos-Induced Cell Injury

Vladimir A. Kostyuk1,*, Alla I. Potapovich1 , Elena N. Vladykovskaya1 , Midori Hiramatsu2
  • 1 Laboratory of Bioenergetics, Byelorussian State University, Minsk, Belarus
  • 2 Institute for Life Support Technology, Yamagata Technopolis Foundation, Yamagata, Japan
Further Information

Publication History

Publication Date:
31 December 2000 (online)

Abstract

Green tea extract was found to provide a strong protective effect against asbestos-induced injury of peritoneal macrophages and red blood cells in vitro. The main polyphenolic constituents of green tea extract, (-)-epicatechin gallate (ECG) and (-)-epigallocatechin gallate (EGCG), were also efficient in preventing injury of cells following exposure to asbestos fibers. The protective efficacies of EGCG and ECG expressed as IC50 values were, respectively, 10 μM and 12 μM if peritoneal macrophages were injured by chrysotile and 4 μM and 5 μM in the case of crocidolite-induced cell injury. Antiradical and chelating properties of ECG and EGCG were evaluated and it was concluded that the protective effect of catechins against asbestos-induced injury may be related to both scavenger properties towards to superoxide anion and the ability to chelate iron ions.

References

  • 1 Rom  W N,, Travis  W D,, Brody  A R.. Cellular and molecular basis of asbestos-related diseases.  Am. Rev. Respir. Dis.. 1991;;  143 408-22
  • 2 Kamp  D W,, Graceffa  P,, Pryor  W A,, Weitzman  S A.. The role of free radicals in asbestos-induced diseases.  Free Radic. Biol. Med.. 1992;;  12 293-315
  • 3 Mossman  B T,, Kamp  D W,, Weitzman  S A.. Mechanisms of carcinogenesis and clinical features of asbestos-associated cancers.  Cancer Invest.. 1996;;  14 (5) 466-80
  • 4 Velichkovsky  B T,, Vladimirov  Y uA,, Korkina  L G,, Suslova  T B.. Physicochemical mechanism of interaction between phagocytic cells and fibrogenic dusts.  Vestn. Akad. Med. Nauk SSSR (Russ.). 1981;;  10 45-51
  • 5 Mossman  B T,, Landesman  J M.. Importance of oxygen-free radicals in asbestos-induced injury to airway epithelial cells.  Chest.. 1983;;  83 50-1
  • 6 Kostyuk  V A,, Potapovich  A I,, Speransky  S D,, Maslova  G T.. Protective effect of natural flavonoids on rat peritoneal macrophages injury caused by asbestos fibers.  Free Radic. Biol. Med.. 1996;;  21 487-93
  • 7 Kostyuk  V A,, Potapovich  A I.. Antiradical and chelating effects in flavonoids protection against silica-induced cells injury.  Arch. Biochem. Biophys.. 1998;;  355 43-8
  • 8 Korkina  L G,, Durnev  A D,, Suslova  T B,, Cheremisina  Z P,, Daugel-Dauge  N O,, Afanas'ev  I B.. Oxygen radical-mediated mutagenic effect of asbestos on human lymphocytes: suppression by oxygen radical scavengers.  Mutat. Res.. 1992;;  265 245-53
  • 9 Yoneda  T,, Hiramatsu  M,, Sakamoto  M,, Togasaki  K,, Komatsu  M,, Yamaguchi  K.. Antioxidant effect of “B-catechin”.  Biochem. Mol. Biol. Int.. 1995;;  35 995-1008
  • 10 Kumary  M VR,, Yoneda  T,, Hiramatsu  M.. Scavenging activity of “B-catechin” on reactive oxygen species generated by photosensitization of riboflavin.  Biochem. Mol. Biol. Int.. 1996;;  38 1163-70
  • 11 Price  M L,, Butler  L G.. Rapid visual estimation and spectrophotometric determination of tannin in sorghum grain.  J. Agric. Food Chem.. 1977;;  25 1268-73
  • 12 Korkina  L G,, Suslova  T B,, Cheremisina  Z P,, Velichkovsky  B T.. Catalytic properties of asbestos fibers and their biological activity.  Stud. Biophys.. 1988;;  126 99-104
  • 13 Beauchamp  C,, Fridovich  I.. Superoxide dismutase improved assay applicable to acrylamide gels.  Anal. Biochem.. 1971;;  44 276-87

Dr. V. A. Kostyuk

Laboratory of Bioenergetics Byelorussian State University

Scorina St. 4

Minsk

220050 Belarus GUS

Email: kostyuk@bio.bsu.unibel.by

Phone: 375 (172) 77-5535