Download PDF
Thromb Haemost 1986; 56(02): 144-146
DOI: 10.1055/s-0038-1661628
Original Article
Schattauer GmbH Stuttgart

IgG Binding to Endothelial Cells in Systemic Lupus Erythematosus

Geneviève Le Roux
1   The Laboratoire d′Hématologie et Departement de Medecine Interne, Hopital Avicenne, Bobigny, France
,
Marie-Paule Wautier
2   The Unite INSERM 150, Hopital Lariboisiere, Paris, France
,
L Guillevin
1   The Laboratoire d′Hématologie et Departement de Medecine Interne, Hopital Avicenne, Bobigny, France
,
J L Wautier
2   The Unite INSERM 150, Hopital Lariboisiere, Paris, France
› Author Affiliations
Further Information

Publication History

Received 06 February 1986

Accepted 27 June 1986

Publication Date:
20 July 2018 (online)

Also available at
  PDF Download  Permissions and Reprints

Summary

Endothelial-asSociated IgG were determined in 20 patients with systemic lupus erythematosis (SLE) - 8 of whom had a lupus anticoagulant (LA) and 6 a history of thrombosis. The binding of IgG present in patient plasma to cultured human endothelial cells was detected using radiolabeled staphylococcal protein A. Thirteen samples gave positive results and a significant association between endothelial-associated IgG and lupus anticoagulant was found (p <0.05). No statistically significant relationship with a previous history of thrombosis was found. These results suggest that the lupus anticoagulant may be directly involved in immune vascular injury induced by either antibodies or immune complexes in SLE.

Key words

SLE - Antiendothelial cells antibodies - Thrombosis and lupus anticoagulant
 

  • References

  • 1 Boey ML, Colaco CB, Gharavi AE, Elkon KB, Loizou S, Hugues GR V. Thrombosis in systemic lupus erythematosus: striking association with the presence of circulating lupus anticoagulant. Br Med J 1983; 287: 1021-1023
    CrossrefPubMedGoogle Scholar
  • 2 Bowie EJ W, Thompson JH, Pascuzzi CA. Thrombosis in systemic lupus erythematosus despite circulating anticoagulants. J Lab Clin Med 1963; 62: 416-430
    PubMedGoogle Scholar
  • 3 Carreras LO, Vermylen JG. "Lupus" anticoagulant and thrombosis. Possible role of inhibition of prostacyclin formation. Thromb Haemos-tas 1982; 48: 38-40
    PubMedGoogle Scholar
  • 4 Elias M, Eldor A. Thromboembolism in patients with the "lupus" -type circulating anti-coagulant. Arch Intern Med 1984; 144: 510-515
    CrossrefPubMedGoogle Scholar
  • 5 Gastineau DA, Kazmier FJ, Nichols WL, Bowie EJ W. Lupus anticoagulant: An analysis of the clinical and laboratory features of 219 cases. Am J Hematol 1985; 19: 265-275
    CrossrefPubMedGoogle Scholar
  • 6 Gladman DD, Urowitz MB, Tozman EC, Glynn MF. Haemostatic abnormalities in systemic lupus erythematosus. Quaterly J Med 1983; Lib 424-433
    PubMedGoogle Scholar
  • 7 Mueh JR, Herbst KD, Rapaport SI. Thrombosis in patients with the lupus anticoagulant. Ann Intern Med 1980; 92: 156-159
    CrossrefPubMedGoogle Scholar
  • 8 Peck B, Hoffman GS, Franck WA. Thrombophlebitis in systemic lupus erythematosus. Jama 1978; 204: 1728-1730
    PubMedGoogle Scholar
  • 9 Carreras LO, Defreyn G, Machin SJ. Arterial thrombosis, intrauterine death and "lupus" anticoagulant: detection of immunoglobulin interfering with prostacyclin formation. Lancet 1981; 1: 244-246
    CrossrefPubMedGoogle Scholar
  • 10 McVerry BA, Machin SJ, Parry H, Goldstone AH. Reduced prostacyclin activity in systemic lupus erythematosus. Ann Rheum Dis 1980; 39: 524
    CrossrefPubMedGoogle Scholar
  • 11 Cariou R, Tobelemyg G. Effet des anticoagulants du type &lupus& sur 1’ activation de la proteine C. Responsabilite dans la survenue des thromboses? 13eme Congres National de Medecine Interne, Dijon, France. 1985 (in press)
    PubMedGoogle Scholar
  • 12 Comp PC, Debault LE, Esmon NL, Esmon CT. Human thrombomodulin is inhibited by IgG from two patients with nonspecific anticoagulants. Blood 1983; (Suppl. 01) 1099
    PubMedGoogle Scholar
  • 13 Cines DB, Lyss AP, Reeber M, Bina M, Dehoratius RJ. Presence of complement fixing anti-endothelial cell antibodies in systemic lupus erythematosus. J Clin Invest 1984; 73: 611-625
    CrossrefPubMedGoogle Scholar
  • 14 Tan EM, Cohen AS, Fries JF. The 1982 revised criteria for the classification of systemic lupus erythematosus. Arthr Rheum 1982; 25: 1271-1277
    CrossrefPubMedGoogle Scholar
  • 15 Kelsey PR, Stevenson KJ, Poller L. The diagnostis of lupus anticoagulants by the activated partial thromboplastin time. The central role of phosphatidyl serine. Thromb Haemostas 1984; 52: 172-175
    PubMedGoogle Scholar
  • 16 Jaffe EA, Nachman RL, Becker CG, Minick CR. Culture of human endothelial cells derived from umbilical veins. Identification by morphologic and immunologic criteria. J Clin Invest 1973; 52: 2745-2756
    CrossrefPubMedGoogle Scholar
  • 17 Howard MA, Firkin BG. Investigations of the lupus-like inhibitor bypassing activity of platelets. Thromb Haemostas 1983; 50: 775-779
    PubMedGoogle Scholar
  • 18 Lafer EM, Rauch J, Andrze-Jewski C. Polyspecific monoclonal lupus auto-antibodies reactive with both polynucleotides and phospholipids. J Exp Med 1981; 153: 897-909
    CrossrefPubMedGoogle Scholar
  • 19 Shoenfeld Y, Rauch J, Massicotte H. Polyspecificity of monoclonal lupus auto antibodies produced by human hybridomas. N Engl J Med 1983; 308: 414-420
    CrossrefPubMedGoogle Scholar
  • 20 Lubbe WF, Butler WS, Palmer SJ, Liggins GC. Lupus anticoagulant in pregnancy. Br J Obstet Gynaecol 1984; 91: 357-363
    CrossrefPubMedGoogle Scholar
  • 21 Prentice RL, Gatenby PA, Loblay RH, Shearman RP, Kronenberg H, Basten A. Lupus anticoagulant in pregnancy. Lancet 1984; 2: 464
    PubMedGoogle Scholar
  • 22 Soulier JP, Boffa MA. Avortements a repetition, thromboses, anticoagulant circulant antithromboplastine. Nouv Presse Med 1980; 9: 859-865
    PubMedGoogle Scholar
  • 23 Harris EN, Asherson RA, Gharavi AE, Morgan SH, Derue G, Hugues GR V. Thrombocytopenia in SLE and related autoimmune disorders: association with anticardiolipin antibody. Br J Haematol 1985; 59: 227-230
    CrossrefPubMedGoogle Scholar
  • 24 Harris EN, Gharavi AE, Boey ML, Patel BM, Mackworth-Young CG, Loizou S, Hugues GR V. Anticardiolipin antibodies: detection by jradioimmuno assay and association with thrombosis in systemic lupus erythematosus. Lancet 1983; 2: 1211-1213
    CrossrefPubMedGoogle Scholar
  • 25 Freyssinet JM, Brami B, Gauchy J, Cazenave JP. Coextraction of thrombomodulin and tissue factor from human placenta: effects of concanavalin A and phospholipid environment on activity. Thromb Haemostas 1986; 55: 112-118
    PubMedGoogle Scholar
  • 26 Angles-Cano E, Sultan Y, Clauvel J. Predisposing factors to thrombosis in systemic lupus erythematosus. J Lab Clin Med 1979; 94: 312-323
    PubMedGoogle Scholar
  • 27 Cines DB, Lyss PA, Bina M, Corkey R, Kefalides NA. Fc and C3 receptors induced by herpes simplex virus on cultured human endothelial cells. J Clin Invest 1982; 69: 123-128
    CrossrefPubMedGoogle Scholar
  • 28 Ryan US, Schultz DR, Ryan JW. Fc and C3b receptors on pulmonary endothelial cells: induction by injury. Science 1981; 214: 557-558
    CrossrefPubMedGoogle Scholar