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Thromb Haemost 1992; 68(03): 364-370
DOI: 10.1055/s-0038-1656381
Original Article
Schattauer GmbH Stuttgart

The Procoagulant Response of Cytomegalovirus Infected Endothelial Cells

M C E van Dam-Mieras
1   The Open University of The Netherlands, Dept. Natural Siences, Heerlen, The Netherlands
,
A D Muller
2   The Limburg University, Dept. Biochemistry, Maastricht, The Netherlands
,
V W M van Hinsbergh
3   The Gaubius Laboratory IVVO-TNO, Leiden, The Netherlands
,
W J H A Mullers
4   The Limburg University, Dept. Medical Microbiology, Maastricht, The Netherlands
,
P H H Bomans
5   The Limburg University, EM Unit, Dept. of Pathology, Maastricht, The Netherlands
,
C A Bruggeman
4   The Limburg University, Dept. Medical Microbiology, Maastricht, The Netherlands
› Author Affiliations
Further Information

Publication History

Received 26 June 1990

Accepted after revision 07 April 1992

Publication Date:
04 July 2018 (online)

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Summary

The report describes the effect of an in vitro infection of human umbilical vein endothelial cells with human Cytomegalovirus (CMV). The parameters studied are cellular procoagulant activity, secretion of plasminogen activator inhibitor (PAI-1) and urokinase-type plasminogen activator (u-PA), activation and internalization of factor X and Merocyanine 540 staining.

The infection does not result in an increase in PAI-1 and u-PA secretion, but it brings about a procoagulant response, which is relatively rapid compared to the tissue factor mediated response induced by inflammatory mediators. The time course and the coagulation factor dependency suggest a facilitated interaction of coagulation factors on the surface of infected cells. Chromogenic activity measurements after the addition of purified factor X and electron microscopic examination of the cells after addition of colloidal gold-factor X conjugates both point to an internalization of factor X and/or Xa after interaction with the endothelial cell surface. Merocyanine 540 staining suggests that CMV infection leads to membrane pertubations.

 

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