Download PDF
Thromb Haemost 1965; 13(01): 155-175
DOI: 10.1055/s-0038-1656222
Originalarbeiten — Original Articles — Travaux Originaux
Schattauer GmbH Schattauer

Kinetic Aspects of the Interaction of Blood Clotting Enzymes

I. Derivation of Basic Formulas
H. C Hemker
1   Haematology Section of the Department of Internal Medicine (Head: Prof .J .Mulder, M.D.), University H ospita I, and the Thrombosis Service (Head: E.A.Loeliger, M.D.), Ley den, The Netherlands.
,
P.W Hemker
1   Haematology Section of the Department of Internal Medicine (Head: Prof .J .Mulder, M.D.), University H ospita I, and the Thrombosis Service (Head: E.A.Loeliger, M.D.), Ley den, The Netherlands.
,
E. A Loeliger
1   Haematology Section of the Department of Internal Medicine (Head: Prof .J .Mulder, M.D.), University H ospita I, and the Thrombosis Service (Head: E.A.Loeliger, M.D.), Ley den, The Netherlands.
› Author Affiliations
Further Information

Publication History

Publication Date:
27 June 2018 (online)

    Permissions and Reprints

Summary

Application of the methods of enzyme-kinetic analysis to the results of clotting tests is feasible and can yield useful results. However, the standard methods of enzyme kinetics are not applicable without modifications imposed by the peculiarities of the blood-clotting enzyme system. The influence of the following complicating circumstances is calculated :

1. Substrate is not present in excess.

2. Only relative measures exist for concentrations of substrate or enzymes.

3. Enzymes and substrates are often added together.

4. Reagents are not pure.

5. Clotting-time is our only measure for clotting-velocity.

Formulas are deduced, which makes it possible to recognize the effect of these complications.

 

  • References

  • 1 Reiner M. Behaviour of enzyme systems. Burgess Publishing Co.; Minneapolis, U. S. A.: 1957
    Google Scholar
  • 2 Dixon M, Webb E. Enzymes. Longmans, Green and Co.; London: 1958
    Google Scholar
  • 3 Report of the International Committee for the Nomenclature of Blood Clotting Factors. 1963. F. K. Schattauer; Stuttgart: 1964
    Google Scholar
  • 4 Report of the Commission on Enzymes of the International Union of Biochemistry. 1961. Pergamon Press; Oxford: 1961
    Google Scholar
  • 5 Lineweaver H, Burk D. The determination of enzyme association constants. J. Amer. ehem. Soc. 56: 658 1934;
    CrossrefPubMedGoogle Scholar
  • 6 Biggs R, Macfariane R. G. Human blood coagulation and its disorders. Blackwell Scientific Publications; Oxford: 3rd ed.. 1962
    Google Scholar
  • 7 Macfariane R. G. An enzyme cascade in the blood clotting mechanism, and its function as a biochemical amplifier. Nature 202: 498 1964;
    CrossrefPubMedGoogle Scholar
  • 8 Schnittger H, Gross R. Über ein Universalgerät zur automatischen Registrierung von Gerinnungszeiten. Klin. Wschr. 32: 1011 1954;
    CrossrefPubMedGoogle Scholar
  • 9 Schnittger H. M. D. Thesis. Marburg 1956
    PubMed
  • 10 Hemker H. C, Veltkamp J. J, Hensen A, Loeliger E. A. Proc. Gleneagles Conf., Internatio -nal Committee for the Nomenclature of Blood Clotting Factors. (1963) Thrombos. Diathes. haemorrh. (Stuttg.) Suppl. 13, p. 380, 1964
    PubMed
  • 11 Hemker H. G, Veltkamp J. J, Hensen A, Loeliger E. A. Nature of prothrombin biosynthesis, preprothrombinaemia in vitamin K-deficiency. Nature 200: 589 1963;
    CrossrefPubMedGoogle Scholar
  • 12 Hemker H. C. Quantitering in de bloedstolling. Maandsch. Kindergeneesk. 22: 503 1964;
    PubMedGoogle Scholar