Pharmacological and Chemical Characteristics of Peanut Extracts Effective in Hemostasis

Summary

Parasympathomimetic activity is exerted by fractions of alcoholic extract of defatted peanuts previously reported effective in hemostasis. The acetylcholine-like effects on isolated tissues may be enhanced by eserine and blocked by atropine. The activity is destroyed by fresh plasma. From studies on active crystalline products obtained by treating the peanut extracts with ammonium reineckate and with potassium triiodide, we conclude that we are working with a mixture of two substances - choline and an ester of choline. We suggest that clinical improvement afforded hemophiliacs by consumptions of defatted peanuts (or extracts thereof) may be attributable to choline.

• References

• 1 Boudreaux H. B, Frampton V. L. A Peanut Factor for Hemostasis in Haemophilia. Nature (Lond.) 180: 469 1960;
• 2 van Creveld S, Mochtar I. A, Koppe J. G. Haemophilia and Therapy with Peanuts, II. Ann. Paediat. (Basel) 202: 1 1964;
• 3 Frampton V. L, Lee L. S, Morris N. J, Boudreaux H. B. Influence of Peanut Extract on Bleeding Time. Thrombos. Diathes. haemorrh. (Stuttg.) 16: 265 1966;
• 4 Macintosh P. G, Perry W. L. M. Biological Estimation of Acetylcholine. In Gerard R. W. ed. Methods in Medical Research. Vol. 3 78 The Year Book Publishers, Inc.; Chicago: 1950
• 5 Booth F. J. A Microchemical Test for Choline and its Esters in Tissue Extracts. Biochem. J 29: 2064 1935;
• 6 Kapfhammer J, Bischojf C. Acetylcholin und Cholin aus tierischen Organen. 1. Darstellung aus Rinderblut. Hoppe-Seylers Z. physiol. Chem 191: 179 1930;
• 7 Boudreaux H. B, Boudreaux R. M, Brandon M, Frampton V. L, Lee L. S. Bioassay of a Hemostatic Factor from Peanuts. Arch. Biochem 89: 276 1960;
• 8 Ghang H. G, Gaddum J. H. Choline Esters in Tissue Extracts. J. Physiol. (Lond.) 79: 255 1933;