Download PDF
Thromb Haemost 1980; 44(01): 030-031
DOI: 10.1055/s-0038-1650074
Original Article
Schattauer GmbH Stuttgart

Labelling of Blood Platelets with Stable Tracers

D Del Principe
1)   The I Department of Pediatrics, University of Rome, Italy
,
R Cesareo
2)   The Istituto di Fisica, Facoltà di Ingegneria, University of Rome, Italy
,
B M Tallarida
1)   The I Department of Pediatrics, University of Rome, Italy
,
M G Ciancarelli
3)   The Cattedra di Fisiologia, L. Istituto di Medicina e Chirurgia, L'Aquila, Italy
,
M Ricci
1)   The I Department of Pediatrics, University of Rome, Italy
,
G Mancuso
1)   The I Department of Pediatrics, University of Rome, Italy
› Author Affiliations
Further Information

Publication History

Received 08 January 1980

Accepted 18 June 1980

Publication Date:
13 July 2018 (online)

  PDF Download  Permissions and Reprints

Summary

Half-time values of platelets labelled with stable rubidium are compared to those of platelets labelled with Cr51. Platelets labelled with stable rubidium are assayed by a very simple version of the X-Ray fluorescence equipment. The mean quantity of rubidium incorporated by the cells is of about some µg Rb per ml blood.

The in vitro half-time of human Rb labelled platelets stored at 22° C is 41.2 ± 3h compared with the value 44.8 ± 3h for platelets labelled with Cr51, as deduced by six experiments. The in vivo half-time of rabbit platelets labelled with stable rubidium is 22 ± 3h compared with the value 18 ± 3h of platelets labelled with Cr51; ten experiments were carried out.

Key words

Blood platelets - Survival; stable tracers
 

  • References

  • 1 Mustard JF. Platelet Survival. Thromb Haemostas 1978; 40: 154-162
    PubMedGoogle Scholar
  • 2 Seltzer RA, Kereiakes JG, Sanger EL. Radiation exposure from radioisotopes in pediatrics. New Engl J Med 1964; 271: 84-90
    CrossrefPubMedGoogle Scholar
  • 3 Stuart MJ, Murphy S, Oski FA. A simple non radioisotope technique for the determination of platelet life span. New Engl J Med 1975; 292: 1310-1313
    CrossrefPubMedGoogle Scholar
  • 4 Cesareo R, Del Principe D, Mancuso G, Tallarida BM. In vitro labelling of platelets with stable Selenocystine. Int J Appl Radiat Isot 1976; 27: 324-326
    CrossrefPubMedGoogle Scholar
  • 5 Cesareo R, Del Principe D, Tallarida BM, Mancuso G. In vitro labeling of platelets with stable Rubidium compounds. Eur J Nucl Med 1978; 3: 91-93
    CrossrefPubMedGoogle Scholar
  • 6 Baldini M, Costea N, Damashek W. The viability of stored human platelets. Blood 1960; 16: 1669-1692
    PubMedGoogle Scholar
  • 7 Cesareo R, Frazzoli FV, Sciuti S. Sensitivity of radioisotope XRF technique with particular regard to portable units. J Radioan Chem 1976; 34: 157-170
    PubMedGoogle Scholar
  • 8 Baadenhuijsen H, De Point JJ H H M, Daemen FJ M. Uptake and efflux of Rb86 by human blood platelets. Biochim Biophys Acta 1973; 298: 690-701
    CrossrefPubMedGoogle Scholar
  • 9 Murphy S, Gardner FH. Platelets preservation: effect of storage temperature on maintenance of platelet viability, deleterious effect of refrigerated storage. New Engl J Med 1969; 280: 1094-1098
    CrossrefPubMedGoogle Scholar
  • 10 Morgan MC, Keating RP, Reisner EH. Survival of radiochromate labelled platelets in rabbits. J Lab Clin Med 1955; 46: 521-530
    PubMedGoogle Scholar
  • 11 Ebbe S, Baldini M, Donovan J. Comparative studies of platelet survival by different methods in the rabbit. Blood 1965; 25: 548-565
    PubMedGoogle Scholar
  • 12 Wistow BW, Grossman ZD, McAfee JG, Subramanian G, Henderson RW, Roskopf ML. Labelling of platelets with Oxine Complexes of Tc-99m and In-111. Part 1. In vitro studies and survival in the rabbit. J Nucl Med 1978; 19: 483-487
    PubMedGoogle Scholar