Download PDF
Thromb Haemost 1994; 72(01): 021-027
DOI: 10.1055/s-0038-1648805
Original Article
Schattauer GmbH Stuttgart

Factor VII Antigen Levels in a Healthy Blood Donor Population

P R Howard
1   The University of Vermont Department of Medical Technology, Burlington, VT, USA
,
E G Bovill
2   Department of Pathology, Vermont Thrombosis Center, Burlington, VT, USA
,
J Pike
3   Department of Biochemistry, Vermont Thrombosis Center, Burlington, VT, USA
,
W R Chruch
3   Department of Biochemistry, Vermont Thrombosis Center, Burlington, VT, USA
,
R P Tracy
2   Department of Pathology, Vermont Thrombosis Center, Burlington, VT, USA
3   Department of Biochemistry, Vermont Thrombosis Center, Burlington, VT, USA
› Author Affiliations
Further Information

Publication History

Received: 26 November 1993

Accepted after resubmission07 March 1994

Publication Date:
12 July 2018 (online)

  PDF Download  Permissions and Reprints

Summary

We determined factor VII antigen (FVIIag) levels in 705 healthy blood donors ranging in age from 17 to 79 years using a two-site solid-phase enzyme immunoassay developed in our laboratory. The mean (± SD) FVIIag level for the total population was 102 ± 31%. FVIIag levels for men (n = 375) and women (n = 330) were 101 ± 28% and 103 ± 33%, respectively. A significant increase in FVIIag was observed with age in both men (r = 0.25, p <0.0001) and women (r = 0.35, p <0.0001). FVIIag levels were significantly higher in women > 60 years when compared to men (median women: 125%; median men: 111%; p <0.05). On a subset of the study group (n = 45), FVIIag levels were correlated to total cholesterol (r = 0.27, p = 0.08) and triglyceride (r = 0.41, p <0.01). Assuming the commonly used reference interval of 60-140% for FVII, the frequencies of FVIIag values for <60% and >140% using our assay were 2.1% and 9.2%, respectively. Gender and age-related differences in FVIIag levels must be considered in a reference interval. We further suggest that assay-specific reference ranges be established, which may include values outside the commonly used values of 60-140%.

 

  • References

  • 1 Carvalho de Sousa J, Bruckert E, Giral P, Soria C, Truffert J, Mirshahi M, de Gennes J, Caen J. Plasma factor VII, triglyceride concentration and fibrin degradation products in primary hyperlipidemia: a clinical and laboratory study. Haemostasis 1989; 19: 83-90
    PubMedGoogle Scholar
  • 2 Dalaker K, Hjermann I, Prydz H. A novel form of factor VII in plasma from men at risk for cardiovascular disease. Br J Haematol 1985; 61: 315-322
    CrossrefPubMedGoogle Scholar
  • 3 Dalaker K, Smith P, Amesen H, Prydz H. Factor VH-phospholipid complex in male survivors of acute myocardial infarction. Acta Med Scand 1987; 222: 111-116
    PubMedGoogle Scholar
  • 4 de Sousa J, Azevedo J, Soria C, Barros F, Ribeiro C, Parreira F, Caen J. Factor VII hyperactivity in acute myocardial thrombosis. A relation to the coagulation activation. Thromb Res 1988; 51: 165-173
    CrossrefPubMedGoogle Scholar
  • 5 Hayes T, Pike J, Tracy R. Factor VII assays. Arch Pathol Lab Med 1993; 117: 52-57
    PubMedGoogle Scholar
  • 6 Hoffman C, Shah A, Sodums M, Hultin M. Factor VII activity state in coronary artery disease. J Lab Clin Med 1988; 111: 475-481
    PubMedGoogle Scholar
  • 7 Hoffman C, Miller R, Lawson W, Hultin M. Elevation of factor VII activity and mass in young adults at risk of ischaemic heart disease. JACC 1989; 14: 941-946
    CrossrefPubMedGoogle Scholar
  • 8 Mann K. Factor VII assays, plasma triglyceride levels and cardiovascular disease risk. Arteriosclerosis 1989; 9: 783-784
    CrossrefPubMedGoogle Scholar
  • 9 Orlando M, Leri O, Macioce G, Mattia G, Ferri G. Factor VII in subjects at risk for thromboembolism: activation or increased synthesis. Haemostasis 1987; 17: 340-343
    PubMedGoogle Scholar
  • 10 Tracy R, Psaty B, Bovill E, Fried L, Heiss G, Lee M, Polak J. Savage P, for tCI. Fibrinogen and factor VIII, but not factor VII, are positively associated with prevalent cardiovascular disease in older adults: analyses from the Cardiovascular Health Study. 32nd Annual Conference on Cardiovascular Disease Epidemiology 1992 Abstract #P31
    PubMedGoogle Scholar
  • 11 Meade T, Brozovic M, Chakrabarti R, Haines A, Imeson J, Mellows S, Miller G, North W, Stirling Y, Thompson S. Haemostatic function and ischaemic heart disease: principal results of the Northwick Park Heart Study. Lancet 1986; 533-537
    PubMedGoogle Scholar
  • 12 Meade T. The epidemiology of haemostatic and other variables in coronary artery disease. In: Thrombosis and Haemostasis. Verstraete M, Vermylen J, Lijnen H, Amout J (eds). Leuven: ISTH and Leuven University Press 1987: 37-59
    PubMedGoogle Scholar
  • 13 Kario K, Matsuo T, Nakao K. Factor VII hyperactivity in the elderly. Thromb Haemostas 1991; 65: 25-27
    PubMedGoogle Scholar
  • 14 Meade T, Imeson J, Haines A, Stirling Y, Thompson S. Menopausal status and haemostatic variables. Lancet 1983; 22-24
    PubMedGoogle Scholar
  • 15 Folsom A, Wu K, Davis C, Conlan M, Sorlie P, Szklo M. Population correlates of plasma fibrinogen and factor VII, putative cardiovascular risk factors. Atherosclerosis 1991; 91: 191-205
    CrossrefPubMedGoogle Scholar
  • 16 Stirling Y, Woolf L, North W, Seghatchian M, Meade T. Haemostasis in normal pregnancy. Thromb Haemost 1984; 52: 176-182
    Article in Thieme ConnectPubMedGoogle Scholar
  • 17 Dalaker K, Prydz H. Coagulation factor VII in pregnancy. Br J Haematol 1984; 56: 233-241
    CrossrefPubMedGoogle Scholar
  • 18 Tracy R, Bovill E, Fried L, Heiss G, Lee M, Polak J, Psaty B, Savage P, for tCI. Coagulation factors in men and women over 65 years: Cardiovascular Health Study. 31st Annual Conference of Cardiovascular Disease Epidemiology 1991 Abstract
    PubMedGoogle Scholar
  • 19 Tracy R, Bovill E. Thrombosis and cardiovascular risk in the elderly. Arch Path Lab Med 1992; 116: 1307-1312
    PubMedGoogle Scholar
  • 20 Miller G, Martin J, Webster J, Wilkes H, Miller N, Wilkinson W, Meade T. Association between dietary fat intake and plasma factor VII coagulant activity - a predictor of cardiovascular mortality. Atherosclerosis 1986; 60: 269-277
    CrossrefPubMedGoogle Scholar
  • 21 Mitropoulos K. Hypercoagulability and factor VII in hypertriglyceridemia. Sem Thromb Hemost 1988; 14: 246-252
    Article in Thieme ConnectPubMedGoogle Scholar
  • 22 Marckmann P, Sandstrom B, Jespersen J. Effect of total fat content and fatty acid composition in diet on factor VII coagulant activity and blood lipids. Atherosclerosis 1990; 80: 227-233
    CrossrefPubMedGoogle Scholar
  • 23 Miller G, Martin J, Mitropoulos K, Reeves B, Thompson R, Meade T, Cooper J, Cruickshank J. Plasma factor VII is activated by postprandial triglyceridemia, irrespective of dietary fat composition. Atherosclerosis 1991; 86: 163-171
    CrossrefPubMedGoogle Scholar
  • 24 Marckmann P, Sandstrom B, Jespersen J. Favorable long-term effect of a low-fat/high-fiber diet on human blood coagulation and fibrinolysis. Arteriosclerosis and Thrombosis 1993; 13: 505-511
    CrossrefPubMedGoogle Scholar
  • 25 Bruckert E, Carvalho de SousaJ, Giral P, Soria C, Chapman M, Caen J, de Gennes J-L. Interrelationship of plasma triglyceride and coagulant factor VII levels in normotriglyceridemic hypercholesterolemia. Atherosclerosis 1989; 75: 129-134
    CrossrefPubMedGoogle Scholar
  • 26 Skartlien A, Lyberg-Beckmann S, Holme I, Hjermenn I, Prydz H. Effect of alteration in triglyceride levels on factor VH-phospholipid complexes in plasma. Arteriosclerosis 1989; 9: 798-801
    CrossrefPubMedGoogle Scholar
  • 27 Tracy R, Losito R, Williams S, Fried L, Furberg C, Kuller L, Robbins J. Plasma levels of coagulation factors II, IX and X, in addition to factor VII, are associated with plasma levels of cholesterol and triglyceride. New York: XlVth Meeting, ISTH 1993
    PubMedGoogle Scholar
  • 28 Hoffman C, Miller R, Hultin M. Correlation of factor VII activity and antigen with cholesterol and triglycerides in healthy young adults. Arteriosclerosis Thrombosis 1992; 12: 267-270
    CrossrefPubMedGoogle Scholar
  • 29 Nakane P, Kawaoi A. Peroxidase-labelled antibody. A new method of conjugation. J Histochem Cytochem 1974; 22: 1084-1091
    CrossrefPubMedGoogle Scholar
  • 30 Litwiller R, Jenny R, Katzmann J, Miller R, Mann K. Monoclonal antibodies to human vitamin K-dependent proteins. Blood 1986; 67: 1583-1590
    PubMedGoogle Scholar
  • 31 Barrowcliffe T. Standardization of factors II, VII, IX, X in plasma and concentrates. Thromb Haemost 1988; 59: 334
    Article in Thieme ConnectPubMedGoogle Scholar
  • 32 Anonymous. Case records of the Massachusetts General Hospital. Weekly clinicopatholgoical exercises. Normal reference laboratory values. N Engl J Med 1992 327. 718-724
    PubMedGoogle Scholar
  • 33 Sasse E, Harris E, Krishnamurthy S, Lee H, Ruland A, Seamonds B. How to define, determine, and utilize reference intervals in the clinical laboratory. NCCLS Documents (C28-P) 1992; 12: 1-47
    PubMedGoogle Scholar
  • 34 Tracy R, Andrianarivo A, Riggs B, Mann K. Comparison of monoclonal and polyclonal antibody-based immunoassays for osteocalcin: a study of sources of variation in assay results. J Bone Min Res 1990; 5: 451-461
    CrossrefPubMedGoogle Scholar
  • 35 Delmas P, Christiansen C, Mann K, Price P. Bone Gla protein (osteocalcin) assay standardization report. J Bone Min Res 1990; 5: 5-11
    CrossrefPubMedGoogle Scholar
  • 36 Fried L, Borhani N, Enright P, Furberg C, Gardin J, Kronmal R, Kuller L, Manolio T, Mittelmark M, Newman A, O’Leary D, Psaty B, Rautaharju P, Tracy R, Weiler P, Group CR. The Cardiovascular Health Study: design and rationale. Ann Epidemiol 1991; 1: 263-276
    CrossrefPubMedGoogle Scholar
  • 37 Mariani G, Mazzucconi M. Factor VII congenital deficiency. Haemostasis 1983; 13: 169-177
    PubMedGoogle Scholar
  • 38 Miletich J, Sherman L, Broze G. Absence of thrombosis in subjects with heterozygous protein C deficiency. N Engl J Med 1987; 317: 991-996
    CrossrefPubMedGoogle Scholar
  • 39 Bovill E, Bauer K, Dickerman J, Callas P, West B. The clinical spectrum of heterozygous protein C deficiency in a large New England kindred. Blood 1989; 73: 712-717
    PubMedGoogle Scholar
  • 40 Tracy R, Bovill E, Fried L, Heiss G, Lee M, Polak J, Psaty B, Savage P. The distribution of coagulation factors VII, VIII and fibrinogen in adults over the age of 65 years: results from the Cardiovascular Health Study. Ann Epidemiol 1992; 2: 509-519
    CrossrefPubMedGoogle Scholar
  • 41 Scarabin P, Van Dreden P, Bonithon-Kop C, Orssauad G, Bara L, Conard J, Samama M. Age-related changes in factor VII activation in healthy women. Clin Sci 1988; 75: 341-343
    CrossrefPubMedGoogle Scholar
  • 42 Scarabin P, Bonithon-Kopp C, Bara L, Malmejac A, Guize L, Samama M. Factor VII activation and menopausal status. Thromb Res 1990; 57: 227-234
    CrossrefPubMedGoogle Scholar
  • 43 Balleisen L, Bailey J, Epping P-H, Schulte H, van de Loo J. Epidemiological study on factor VII, factor VIII and fibrinogen in an industrial population: I. Baseline data on the relation to age, gender, body-weight, smoking, alcohol, pill-using, and menopause. Thromb Haemost 1985; 54: 475-479
    Article in Thieme ConnectPubMedGoogle Scholar
  • 44 Boyer C, Wolf M, Rothchild C, Migaud M, Amiral J, Mannucci P, Meyer D, Larrieu M. An enzyme immunoassay (ELISA) for the quantitation of human factor VII. Thromb Haemost 1986; 56: 250-255
    Article in Thieme ConnectPubMedGoogle Scholar
  • 45 Fair D. Quantitation of factor VII in the plasma of normal and warfarin-treated individuals by radioimmunoassay. Blood 1983; 62: 784-791
    PubMedGoogle Scholar
  • 46 Mikami S, Tuddenham E. Studies on an immunological assay of vitamin K-dependent factors. Measurement of factor VII antigen by radioimmunoassay. Brit J Haematol 1986; 62: 171-181
    CrossrefPubMedGoogle Scholar