Thromb Haemost 1992; 67(01): 182
DOI: 10.1055/s-0038-1648404
Letters to the Editor
Schattauer GmbH Stuttgart

Cytoplasmic Localization of Aequorin Loaded into Human Platelets by a New Method

Masahiro Saitoh
Cardiovascular Division, Department of Medicine and the Department of Surgery, Beth Israel Hospital and Department of Cellular and Molecular Physiology, Harvard Medical School, Boston, MA 02215, USA
,
Henry S Slayter
Cardiovascular Division, Department of Medicine and the Department of Surgery, Beth Israel Hospital and Department of Cellular and Molecular Physiology, Harvard Medical School, Boston, MA 02215, USA
,
Simon C Watkins
Cardiovascular Division, Department of Medicine and the Department of Surgery, Beth Israel Hospital and Department of Cellular and Molecular Physiology, Harvard Medical School, Boston, MA 02215, USA
,
J Anthony Ware
Cardiovascular Division, Department of Medicine and the Department of Surgery, Beth Israel Hospital and Department of Cellular and Molecular Physiology, Harvard Medical School, Boston, MA 02215, USA
› Author Affiliations
Further Information

Publication History

Received 15 May 1991

Accepted after revision 18 July 1991

Publication Date:
02 July 2018 (online)

 
  • References

  • 1 Johnson PC, Ware JA, Cliveden PB, Smith M, Dvorak AM, Salzman EW. Measurement of ionized calcium in blood platelets with the photoprotein aequorin: Comparison with Quin 2. J Biol Chem 1985; 260: 2069-2076
  • 2 Ware JA, Johnson PC, Smith M. Salzman EW Effects of common agonists on cytoplasmic ionized calcium concentration in platelets. J Clin Invest 1986; 77: 878-886
  • 3 Lages B, Weiss HJ. Time dependence of aequorin-indicated calcium levels in stimulated and unstimulated platelets: Evidence for multiple aequorin environments in platelets. Thromb Haemostas 1989; 62: 1094
  • 4 Snowdowne KW, Borle AB. Measurement of cytoplasmic free calcium in mammalian cells with aequorin. Am J Physiol 1984; 246 (Cell Physiol 16) C396-408
  • 5 Yamaguchi A, Suzuki H, Tanoue K, Yamazaki H. Simple method of aequorin loading into platelets using dimethyl sulfoxide. Thromb Res 1986; 44: 165-174
  • 6 Watkins SC, Samuel JL, Marotte F, Bertier-Savalle B, Rappaport L. Microtubules and desmin filaments during onset of heart hypertrophy in rat: a double immuno electron microscopic study. Circ Res 1987; 60: 327-336