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Thromb Haemost 1979; 41(04): 787-795
DOI: 10.1055/s-0038-1646838
Original Articles
Schattauer GmbH Stuttgart

Abnormalities of Platelet Adenine Nucleotides in Patients with Myeloproliferative Disorders

Junji Nishimura
The Third Department of Internal Medicine, Faculty of Medicine, Kyushu University, Fukuoka 812, Japan
,
Seiki Okamoto
The Third Department of Internal Medicine, Faculty of Medicine, Kyushu University, Fukuoka 812, Japan
,
Hiroshi Ibayashi
The Third Department of Internal Medicine, Faculty of Medicine, Kyushu University, Fukuoka 812, Japan
› Author Affiliations
Further Information

Publication History

Received 14 April 1978

Accepted 12 August 1978

Publication Date:
09 July 2018 (online)

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Summary

Platelet aggregation and adenine nucleotides in platelets have been studied in thirteen patients with myeloproliferative disorders. ADP induced aggregation was abnormal in two patients, but collagen induced aggregation was impaired in 11 patients. The concentrations of ATP and ADP in resting platelets in the patients with abnormal aggregation were significantly less than those in normal subjects. Marked reduction of the amounts of both nucleotides released into plasma was also observed after stimulation of collagen in these patients. Platelets in the patients with normal functions contained almost normal amounts of adenine nucleotides. We discussed the relationship between platelet dysfunction and adenine nucleotides in platelets of myeloproliferative disorders and concluded that platelet dysfunction was mainly attributable to reduction of releasable ADP.

 

  • References

  • 1 Berger S, Aledort LM, Gilbert HS, Hanson JP, Wasserman LR. 1973; Abnormalities of platelet function in patients with polycythemia vera. Cancer Research 33: 2683
    PubMedGoogle Scholar
  • 2 Born GV R, Cross MJ. 1963; The aggregation of blood platelets. Journal of Physiology 168: 178
    CrossrefPubMedGoogle Scholar
  • 3 Boughton BJ, Corbett WE N, Ginsburg AD. 1977; Myeloproliferative disorders, a paradox of in-vivo and in-vitro platelet function. Journal of Clinical Pathology 30: 228
    CrossrefPubMedGoogle Scholar
  • 4 Cardamone JM, Edson JR, Mcarthur JR, Jacob HS. 1972; Abnormalities of platelet function in the myeloproliferative disorders. Journal of American Medical Association 221: 270
    CrossrefPubMedGoogle Scholar
  • 5 Chievitz E, Thiede C. 1962; Complications and causes of death in polycythemia vera. Acta Medica Scandinavica 172: 513
    PubMedGoogle Scholar
  • 6 Cowan DH, Graham Jr RC, Baunach D. 1975; The platelet defect in leukemia, platelet ultrastructure, adenine nucleotide metabolism, and the release reaction. Journal of Clinical Investigation 56: 188
    CrossrefPubMedGoogle Scholar
  • 7 Ginsburg AD. 1975; Platelet function in patients with high platelet counts. Annals of Internal Medicine 82: 506
    CrossrefPubMedGoogle Scholar
  • 8 Gunz FW. 1960; Hemorrhagic thrombocythemia. Blood 15: 706
    PubMedGoogle Scholar
  • 9 Hamberg M, Svensson J, Samuelsson B. 1975; Thromboxanes, a new group of biologically active compounds derived from prostaglandin endoperoxides. Proceedings of the National Academy of Sciences of the United States of America 72: 2992
    PubMedGoogle Scholar
  • 10 Han P, Ardlie NG. 1974; The influence of pH, temperature, and calcium on platelet aggregation, maintenance of enviromental pH and platelet function for in vitro studies in plasma stored at 37°C. British Journal of Haematology 26: 373
    CrossrefPubMedGoogle Scholar
  • 11 Holmsen H, Day HJ, Storm E. 1969; Adenine nucleotide metabolism of blood platelets VI. Subcellular localization of nucleotide pools with different functions in the platelet release reaction Biochimica et Biophysica Acta 186: 254
    CrossrefPubMedGoogle Scholar
  • 12 Holmsen H, Weiss HJ. 1970; Hereditary defect in the platelet release reaction caused by a deficiency in the storage pool of platelet adenine nucleotides. British Journal of Haematology 19: 643
    CrossrefPubMedGoogle Scholar
  • 13 Huzoor-Akbar Ardlie NG. 1976; Evidence that collagen releases human platelet constituents by two different mechanisms. British Journal of Haematology 34: 137
    CrossrefPubMedGoogle Scholar
  • 14 Keenan JP, Wharton J, Shepherd AJ N, Bellingham AJ. 1977; Defective platelet lipid peroxidation in myeloproliferative disorders, a possible defect of prostaglandin synthesis. British Journal of Haematology 35: 275
    CrossrefPubMedGoogle Scholar
  • 15 Mason Jr JE, Devita VT, Canellos GP. 1974; Thrombocytosis in chronic granulocytic leukemia, incidence and clinical significance. Blood 44: 483
    PubMedGoogle Scholar
  • 16 Neemeh JA, Bowie EJ W, Thompson Jr JH, Didisheim P, Owen CA. 1972; Quantitation of platelet aggregation in myeloproliferative disorders. American Journal of Clinical Pathology 57: 336
    CrossrefPubMedGoogle Scholar
  • 17 Nishimura J, Minakami S. 1975; Effect of hydrogen ion concentration on energy metabolism in pig platelets. Biochimica et Biophysica Acta 396: 1
    CrossrefPubMedGoogle Scholar
  • 18 Nurden AT, Caen JP. 1974; An abnormal platelet glycoprotein pattern in three cases of Glanzmann’s thrombasthenia. British Journal of Haematology 28: 253
    CrossrefPubMedGoogle Scholar
  • 19 Okuma M, Uchino H. 1977; Platelet lipoxygenase deficiency. New England Journal of Medicine 297: 1351
    PubMedGoogle Scholar
  • 20 Penington DG, Streatfield K. 1975; Heterogeneity of megakaryocytes and platelets. Series Haematologica 8: 22
    PubMedGoogle Scholar
  • 21 Spaet TH, Bauer D, Melamed S. 1956; Haemorrhagic thrombocythemia, a blood coagulation disorder. Archives of Internal Medicine 98: 377
    CrossrefPubMedGoogle Scholar
  • 22 Spaet TH, Lejnieks I, Gaynor E, Goldstein ML. 1969; Defective platelets in essential thrombocythemia. Archives of Internal Medicine 124: 135
    CrossrefPubMedGoogle Scholar
  • 23 Zucker S, Mielke CH. 1972; Classification of thrombocytosis based on platelet function tests, correlation with hemorrhagic and thrombotic complications. Journal of Laboratory and Clinical Medicine 80: 385
    PubMedGoogle Scholar