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Thromb Haemost 2001; 86(02): 680-685
DOI: 10.1055/s-0037-1616104
Review Article
Schattauer GmbH

Glucose and Insulin Modulate the Capacity of Endothelial Cells (HUVEC) to Express P-selectin and Bind a Monocytic Cell Line (U937)

Marta D. Puente Navazo
1   Centre Pluridisciplinaire d’Oncologie, ISREC, Epalinges, Switzerland
,
Kamal Chettab
1   Centre Pluridisciplinaire d’Oncologie, ISREC, Epalinges, Switzerland
,
Jacques Duhault
2   Institut de Recherche Servier, Suresnes, France
,
Elisabeth Koenig-Berard
2   Institut de Recherche Servier, Suresnes, France
,
John L. McGregor
3   INSERM Unit 331, Faculté de Medicine RTH Laënnec, Université Claude Bernard, Lyon, France
› Author Affiliations
Further Information

Publication History

Received 03 July 2000

Accepted after resubmission 16 March 2001

Publication Date:
12 December 2017 (online)

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Summary

Diabetes mellitus is associated with increased prevalence of endothelial cell dysfunction and vascular diseases. Mechanisms leading to alterations in endothelial cell function are poorly understood. We report here that hyperglycaemia results in the expression of endothelial adhesion molecules involved in leukocyte adhesion and extravasation. Incubation of human umbilical cord endothelial cells (HUVEC) with 25 mM glucose induced the expression of P-selectin. This effect was reversed by the addition of 1 nM insulin. Moreover, increased ICAM-1 expression was observed upon HUVEC incubation with 25 mM glucose. Increased adhesion of U937 cells (a monocytic cell line) to endothelial cells cultured with 25 mM glucose was observed. High glucose-induced monocytes cell adhesion was inhibited by an anti-P-selectin monoclonal antibody (LYP20). These results show that high glucose concentration activates endothelial cells leading to monocytes adhesion providing further evidence that hyperglycaemia might be implicated in vessel wall lesions contributing to diabetic vascular disease.

Present address: Dr. M. D. Puente Navazo, Centre Pluridisciplinaire d’Oncologie, ISREC, Epalinges, Switzerland

Keywords

Endothelial cells - P-selectin - hyperglycaemia
 

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