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Thromb Haemost 2001; 86(05): 1320-1326
DOI: 10.1055/s-0037-1616067
Review Article
Schattauer GmbH

Increased Expression of Serine Palmitoyltransferase (SPT) in Balloon-injured Rat Carotid Artery

David J. Uhlinger
1   R.W. Johnson Pharmaceutical Research Institute, Raritan, New Jersey
,
Jill M. Carton
2   Department of Molecular and Cellular Biology, Centocor, Inc., Malvern, PA
,
Dennis C. Argentieri
1   R.W. Johnson Pharmaceutical Research Institute, Raritan, New Jersey
,
Bruce P. Damiano
3   Spring House, Pennsylvania, USA
,
Michael R. D’Andrea
3   Spring House, Pennsylvania, USA
› Author Affiliations
Further Information

Publication History

Received 15 February 2001

Accepted after resubmission 19 June 2001

Publication Date:
13 December 2017 (online)

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Summary

The response to vascular injury is a complex wound healing response involving cell proliferation, migration, remodeling and inflammation. In the present studies we employed a rat balloon angioplasty model of vascular injury to investigate the potential role of sphingolipid signaling in the response to vascular injury. The enzyme serine palmitoyltransferase (SPT) catalyzes the first committed step in de novo sphingolipid biosynthesis. We observed marked upregulation of expression of both SPT subunits in actively proliferating cells in injured vessels. This enhanced SPT expression occurs in de-differentiated fibroblasts and proliferating vascular smooth muscle cells. The upregulation is particularly apparent in the proliferating luminal edge of the neointima and the adventitial de-differentiated fibroblasts and may serve as a hallmark of this process. The possible functional consequences of this enzyme upregulation and its role in the response to vascular injury are suggested but remain to be determined.

Keywords

Serine palmitoyltransferase -1 and -2 - rat balloon angioplasty - restenosis - immunohistochemistry - reactive fibroblasts
 

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