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Thromb Haemost 2001; 85(02): 314-319
DOI: 10.1055/s-0037-1615686
Review Article
Schattauer GmbH

β2-glycoprotein I Binding to Platelet Microparticle Membrane Specifically Reduces Immunoreactivity of Glycoproteins IIb/IIIa

Laurent Vallar
1   Laboratoire Franco-Luxembourgeois de Recherche Biomédicale (CNRS and CRP-Santé), Centre Universitaire, Luxembourg
,
Véronique Regnault
2   Laboratoire d’Hématologie, UMR CNRS 7563, Faculté de Médecine, Vandoeuvre-lès-Nancy, France
,
Véronique Latger-Cannard
2   Laboratoire d’Hématologie, UMR CNRS 7563, Faculté de Médecine, Vandoeuvre-lès-Nancy, France
3   Hématologie Biologique, CHU, Nancy, France
,
Thomas Lecompte
2   Laboratoire d’Hématologie, UMR CNRS 7563, Faculté de Médecine, Vandoeuvre-lès-Nancy, France
3   Hématologie Biologique, CHU, Nancy, France
› Author Affiliations
Further Information

Publication History

Received 22 February 2000

Accepted after resubmission 25 September 2000

Publication Date:
08 December 2017 (online)

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Summary

We have investigated β2-glycoprotein I (β2GPI) binding to platelet-derived microparticles (PMP) and its effect on GPIIb/IIIa. PMP were isolated from washed human platelets after stimulation with A23187, and analyzed by surface plasmon resonance spectroscopy. β2GPI as well as activated protein C (APC) or annexin V bound to PMP-coated sensorchips, demonstrating exposure of anionic phospholipids on immobilized PMP. β2GPI binding was impaired by calcium and occurred in a concentration-dependent manner with apparent kon = 2.6104 M-1.s-1 and koff = 4.410-3 s-1, corresponding to a KD value of 1.710-7 M. When analyzed by flow cytometry, the binding of certain mAbs specific for GPIIb and/or GPIIIa was reduced in the presence of β2GPI but not of APC or annexin V, whereas the binding of anti-GPIb or anti-P-selectin mAbs, or of soluble fibrinogen remained unchanged. These results suggest a broad but specific influence of β2GPI on GPIIb/IIIa immunoreactivity, and indicate that β2GPI may act as a modulator of GPIIb/IIIa-dependent functions of PMP.

Keywords

β2-glycoprotein I - platelet microparticles - glycoproteins IIb/IIIa
 

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