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Int J Sports Med 2010; 31(12): 847-853
DOI: 10.1055/s-0030-1265205
Physiology & Biochemistry

© Georg Thieme Verlag KG Stuttgart · New York

Protective Effect of Erythropoietin on Renal Injury Induced by Acute Exhaustive Exercise in the Rat

X. Lin1 , S. Qu1 , M. Hu2 , C. Jiang1 , 3
  • 1Medical College of Hunan Normal University, Changsha, China
  • 2Public Health College of Central South University, Changsha, China
  • 3University of Linköping, Department of Clinical and Experimental Medicine, Sweden
Further Information

Publication History

accepted after revision August 23, 2010

Publication Date:
11 November 2010 (online)

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Abstract

We investigated the protective effect of Erythropoietin (EPO) analogue rHuEPO on renal injury induced by acute exhaustive exercise in the rat. Rats were randomly allocated to one of 3 groups: normal control (C), exhaustive exercise test (ET) and EPO pre-treatment (rHuEPO 2 000 U/kg) plus ET (EPO+ET). Compared with controls, animals in the ET group had increased serum urea nitrogen, serum creatinine, urine protein, and renal tissue malondialdehyde (MDA) and decreased renal tissue nitric oxide (NO), nitric oxide synthase (NOS) and superoxide dismutase (SOD) activities. There was severe damage in renal tubular epithelial cells with a lot of cell apoptosis, and TUNEL assay revealed a remarkably high apoptotic index (p<0.01). Changes in renal function and kidney tissue were much less in the EPO+ET group (p<0.05) and the apoptotic index was much lower than in the ET group (18.45±0.32 vs. 27.55±0.49, p<0.05). EPO pretreatment thus significantly prevented renal cell apoptosis, and counteracted high MDA and low NO and NOS renal contents induced by exhaustive exercise. The data point to a potential value of EPO in preventing the acute renal injury after exhaustive exercise.

Key words

erythropoietin - exhaustive exercise - apoptosis - kidney - rat

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Correspondence

Prof. Shulin Qu

Medical College of Hunan

Normal University

Department of Physiology

Tongzipo Road 371

410013 Changsha

China

Phone: 008673188630333

Fax: 008673188630333

Email: qushulin07@yahoo.com.cn

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