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Exp Clin Endocrinol Diabetes 1994; 102(5): 364-369
DOI: 10.1055/s-0029-1211305
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© J. A. Barth Verlag in Georg Thieme Verlag KG Stuttgart · New York

Expression of insulin-like growth factor 1 (IGF-1) in the bovine oviduct during the oestrous cycle

A. Schmidt1 , R. Einspanier1 , W. Amselgruber2 , F. Sinowatz2 , D. Schams1
  • 1Institut für Physiologie, TU München, Freising-Weihenstephan, Germany
  • 2Institut für Anatomie II, LMU München, Germany
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Publication History

Publication Date:
15 July 2009 (online)

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Summary

Growth factors are known to take part in the regulation of reproduction. Here we present evidence for the expression of insulin-like growth factor 1 (IGF-1) in the bovine oviduct. Two IGF-1 specific mRNA-tran-scripts of 1.5 and 4.4 kb were detected during the whole oestrous cycle, and showed increased expression after ovulation. Complete homology to the known IGF-1 sequence was achieved by specific RT-PCR (reverse transcription — polymerase chain reaction) amplification followed by dideoxysequencing of this 210 bp fragment. IGF-1 protein was localized in the secretory cells of the oviduct epithelium using immunohistochemical techniques. We suggest possible effects of IGF-1 during ovulation either on the oviductal cells or on the early embryo.

Key words

IGF-1 - mRNA - cattle - oviduct

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