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Exp Clin Endocrinol Diabetes 1990; 95(2): 229-236
DOI: 10.1055/s-0029-1210957
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© J. A. Barth Verlag in Georg Thieme Verlag KG Stuttgart · New York

A Sensitive Sandwich Enzyme Immunoassay for Measurement of Insulin on Microtiter Plates

J. Kratzsch, W. Ackermann, H. Keilacker, W. Besch, E. Keller
  • Institute of Clinical Chemistry and Laboratory Diagnosis (Director : MR Prof. Dr. sc. med. W. Rotzsch) and Department of Pediatrics (Director : OMR Prof. Dr. sc. med. W. Braun), Karl-Marx-University Medical School Leipzig, Central Institute of Diabetes “Gerhardt Katsch” (Director : OMR Prof. Dr. sc. med. H. Bibergeil) Karlsburg/GDR
Further Information

Publication History

1989

Publication Date:
16 July 2009 (online)

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Summary

A sensitive enzyme immunoassay for the measurement of insulin in human sera on microtiter plates was established. The assay is based on the sandwich technique with guinea pig anti-insulin IgG adsorbed at microtiter plate wells, human insulin as standard and the same anti-insulin IgG labeled with horseradish peroxidase. Standards used cover a range from 0 to 1200pmol/l with a detection limit of 10 pmol/1. Coefficients of variation between 3—7% for intraassay precision and 5—11% for interassay precision were obtained over the concentration range of 80—1000 pmol/1. The correlation of EIA-data with those of a commercially available double antibody radioimmuno-assay (r = 0.98) could be expressed by the equation : EIA = 0.97 RIA — 57 pmol/1. Normal fasting serum insulin concentrations in healthy subjects ranged from 11—165 pmol/1. In subjects with potentially diminished basal values concentrations of 10—79 pmol/1 were determined. The insulin response in oral glucose tolerance tests of children was discussed, who had a constitutional tall stature or Turner's syndrom, respectively.

Key words

Insulin determination - Enzyme immunoassay - Microtiter plates - Serum insulin concentrations