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Exp Clin Endocrinol Diabetes 1986; 87(1): 69-78
DOI: 10.1055/s-0029-1210525
Original

© J. A. Barth Verlag in Georg Thieme Verlag KG Stuttgart · New York

The Action of Cyclosporin A on the Endocrine Pancreas in Vitro - Functional and Morphological Studies

F. Laube, W. Blech, D. Schröder, W. Besch, W. Hildebrandt, H. J. Hahn
  • Central Institute of Diabetes (Head: OMR Prof. Dr. H. Bibergeil) Karlsburg and Institute of Biochemistry (Head: Prof. Dr. H. Aurich) Martin-Luther-University Halle/GDR
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Publication History

1985

Publication Date:
16 July 2009 (online)

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Summary

We investigated the effect of Cyclosporin A (100 ng/ml — 12000 ng/ml) on functional parameters of the endocrine pancreas in vitro. The insulin secretion of isolated islets in response to a variety of secretory stimulators is characterized by minimal alteration occasionally observed in the presence of very high Cyclosporin A concentrations. Cyclosporin A did not alter the glucose-induced biphasic insulin secretion, nor the arginine-stimulated glucagon secretion of the perfused rat pancreas. Exposure of isolated islets to 2 000 ng Cyclosporin A/ml for 3 weeks resulted in a minimal decrease of insulin content and a marked reduction of glucagon content, which were not accompanied by drastic alterations of insulin secretion. Scanning electron micrographs of these Cyclosporin A-treated islets did not differ from cultured control islets. The results let us assume that Cyclosporin A exerts no toxic effects on the endocrine pancreas in vitro, when exposed to concentrations ≥2000 ng/ml.

Key words

Isolated islet - Insulin secretion - Glucagon secretion - Culture - Cyclosporin A

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