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Planta Med 2010; 76(3): 223-227
DOI: 10.1055/s-0029-1186055
Pharmacology
Original Papers
© Georg Thieme Verlag KG Stuttgart · New York

Enteric Mucosal Immune Response might Trigger the Immunomodulation Activity of Ganoderma lucidum Polysaccharide in Mice

Hongyan Zhao1 , Yingying Luo2 , Cheng Lu3 , Na Lin4 , Cheng Xiao5 , Shuhong Guan6 , De-an Guo6 , Zhenli Liu2 , Dahong Ju1 , Xiaojuan He3 , Aiping Lu3 , 7
  • 1Institute of Basic Theory, China Academy of Chinese Medical Sciences, Beijing, P. R. China
  • 2Jiangxi University of Traditional Chinese Medicine, Nanchang, Jiangxi, P. R. China
  • 3Institute of Basic Research in Clinical Medicine, China Academy of Chinese Medical Sciences, Beijing, P. R. China
  • 4Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing, P. R. China
  • 5Institute of Clinical Medicine, China-Japan Friendship Hospital, Beijing, P. R. China
  • 6Shanghai Research Center for TCM Modernization, Shanghai Institute of Materia Medica, Shanghai Institute for Biological Sciences, Chinese Academy of Siciences, Shanghai, P. R. China
  • 7E-institute of Shanghai Municipal Education Commission, Shanghai TCM University, Shanghai, P. R. China
Further Information

Publication History

received February 19, 2009 revised June 15, 2009

accepted July 24, 2009

Publication Date:
25 August 2009 (online)

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Abstract

Ganoderma lucidum is a well-known traditional Chinese medicine and often used for improving quality of life. Ganoderma lucidum polysaccharide (GLP) is the main active component in the fungus. Recent researches have shown that oral administration of GLP could produce immunomodulation and antitumor activity. With a high molecular weight, GLP is not easy to be absorbed, and the pathway via enteric mucosal immune response might be important for the immunomodulation of GLP. To investigate the potential mechanism, Kunming mice, weighing 20 ± 2 g, were used and their peripheral blood mononuclear cells (PBMC), intraepithelial lymphocytes (IEL) and Peyer's patches lymphocytes (PPL) were separated, and incubated with GLP at different dosages (250 µg/mL, 125 µg/mL, 62.5 µg/mL, 31.25 µg/mL). MTT, ELISA and RT‐PCR were used to detect the effects of GLP on the proliferation of the lymphocytes, and expression of IL-10, IL-2 and TNF-α. The results showed that GLP could stimulate the proliferation of PBMC and enteric mucosal lymphocytes, and promote the production of IL-2 and IL-10. The RT‐PCR results also showed a higher expression of TNF-α and IL-10 mRNA in the lymphocytes. The higher expression of IL-2, IL-10 and TNF-α from the lymphocytes was observed at GLP concentrations of 62.5 µg/mL and 125 µg/mL. In conclusion, enteric mucosal immune responses could be one of the important pathways for the immunomodulation activity of GLP.

Key words

mucosal immune response - cytokines - Ganoderma lucidum polysaccharide - Ganoderma lucidum - Polyporaceae

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PhD, MD Aiping Lu

Institute of Basic Research in Clinical Medicine
China Academy of Chinese Medical Sciences

No. 16, Nanxiaojie, Dongzhimennei

Beijing 100700

People's Republic of China

Phone: + 86 10 64 06 76 11

Fax: + 86 10 64 06 76 11

Email: lap64067611@126.com

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