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Horm Metab Res 1970; 2(3): 161-165
DOI: 10.1055/s-0028-1095086
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© Georg Thieme Verlag KG Stuttgart · New York

Physiologic and Genetic Influences on Regulation of Glucose Metabolism in Adipose Tissue of Mice

Terence T. T. Yen , L.  Lowry , J.  Steinmetz , G. L. Wolff [*]
  • Biological Research, The Lilly Research Laboratories, Eli Lilly and Company, Indianapolis, Indiana, and The Institute for Cancer Research, Philadelphia, Pennsylvania, USA
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Publication Date:
08 January 2009 (online)

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Abstract

The influence of various agouti locus genotypes and phenotypes, castration, fat content of the diet and their interactions on the rates of glucose oxidation and conversion to lipid, blood glucose level, epididymal adipose tissue weight and body weight has been studied in male mice of the inbred YS/ChWf, VY/Wf, C3H/DiHeWf-Avy and NZO/BlWf strains. Although the lethal yellow (Ay) and viable yellow (Avy) genes, castration and a high fat content diet, all increased epididymal adipose tissue weight, the inducing mechanisms are probably different for each of these factors.

Body weight of agouti Avy/a mice was less than that of their genetically identical mottled yellow Avy/a brothers. Their rates of glucose oxidation and conversion to lipid were higher and resembled the rates among comparable a/a mice rather than those among mottled yellow Avy/a mice.

Among the parameters measured, the rate of glucose oxidation appeared to be correlated only with body weight. This correlation was physiologic and not genetic. The rate of conversion of glucose to lipid was not correlated directly with any parameters measured.

Intact Ay/a and a/a YS mice had similar rates of glucose utilization. However, castration revealed a difference in metabolic regulation of glucose metabolism between the genotypes. Rates of glucose oxidation and conversion to lipid were significantly reduced in Ay/a mice by castration while no effect was observed in the a/a mice.

The difference in diet fat content exerted no effect on the conversion rate, of glucose to lipid in New Zealand Obese (NZO) mice although this rate was reduced by the 11% fat diet in intact Ay/a YS mice.

Key words

Adipose Tissue Metabolism - Glucose Oxidation - Agouti Locus - Yellow Obese Mice - New Zealand Obese Mice - Lethal Yellow Gene - Viable Yellow Gene - Obesity - Castration

1 The portion of the work performed by G.L.W. at The Institute for Cancer Research has been supported in part by U.S.P.H.S. grants CA-06927 and FR-05539 from the National Institutes of Health and by an appropriation from the Commonwealth of Pennsylvania.