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Horm Metab Res 1976; 8(3): 202-206
DOI: 10.1055/s-0028-1093660
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© Georg Thieme Verlag KG Stuttgart · New York

Cyclic AMP Level of Human Thyroid Cells in Monolayer Culture TSH Induced Refractoriness to TSH Action

Y.  Kaneko
  • The First Department of Medicine, University of Tokyo, Hongo, Tokyo, Japan
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Publication History

Publication Date:
23 December 2008 (online)

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Abstract

Thyroid cells from euthyroid patients with Graves' disease were cultured in a chemically defined medium. The cells preserved the ability to respond to TSH with 8-fold increase in cyclic AMP concentration. This cyclic AMP response to TSH was diminished by prior exposure of cells to TSH. The decrease in cyclic AMP response to TSH induced by TSH was reversible, was not associated with a similar decrease in cyclic AMP response to PGE1, and could not be attributed to increased phosphodiesterase activity or to decreased adenyl cyclase activity. The partial resistence to TSH stimulation of thyroid cells previously exposed to TSH may be due to changes in the TSH receptor, possibly caused by TSH itself.

Key words

Thyroid Cells - Cell Culture - cAMP - TSH - PGE1