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Synfacts 2008(11): 1213-1213
DOI: 10.1055/s-0028-1083392
DOI: 10.1055/s-0028-1083392
Organo- and Biocatalysis
© Georg Thieme Verlag
Stuttgart ˙ New York
Synthesis of (R)-Pantolactone
B. Pscheidt, Z. Liu, R. Gaisberger, M. Avi, W. Skranc, K. Gruber, H. Griengl, A. Glieder*
Research Centre Applied Biocatalysis GmbH, Graz, Graz University of Technology, and DSM Fine Chemicals Austria Nfg. GmbH & Co KG, Linz, Austria
Further Information
Publication History
Publication Date:
23 October 2008 (online)
Significance
A pH-stable hydroxynitrile lyase (HNL) for the conversion of hydroxypivaldehyde (1) and pivaldehyde was developed by screening for stereoselective cyanohydrin synthesis in 96 well plates. Stepwise redesign of PaHNL5 gene and enzyme leads to elevated amounts of a highly efficient biocatalyst for (R)-pantolactone (2) synthesis (er = 98.5:1.5, 100% conversion) .