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Planta Med 2020; 86(06): 387-394
DOI: 10.1055/a-1129-7026
Biological and Pharmacological Activity
Original Papers
Georg Thieme Verlag KG Stuttgart · New York

Inhibitory Activity of Extract, Fractions, and Compounds from Zingiber montanum Rhizomes on the Migration of Breast Cancer Cells

Mohammad Al-Amin
1   Discipline of Pharmaceutical Chemistry, School of Pharmaceutical Sciences, Universiti Sains Malaysia, Penang, Malaysia
,
Nagla Mustafa Eltayeb
1   Discipline of Pharmaceutical Chemistry, School of Pharmaceutical Sciences, Universiti Sains Malaysia, Penang, Malaysia
2   Tropical Medicine Research Institute (TMRI), National Centre for Research (NCR), Khartoum, Sudan
,
Chowdhury Faiz Hossain
3   Department of Pharmacy, East West University, Aftabnagar, Dhaka, Bangladesh
,
Melati Khairuddean
4   School of Chemical Sciences, Universiti Sains Malaysia, Penang, Malaysia
,
Siti Sarah Fazalul Rahiman
5   Discipline of Physiology, School of Pharmaceutical Sciences, Universiti Sains Malaysia, Penang, Malaysia
,
Salizawati Muhamad Salhimi
1   Discipline of Pharmaceutical Chemistry, School of Pharmaceutical Sciences, Universiti Sains Malaysia, Penang, Malaysia
› InstitutsangabenGefördert durch: Universiti Sains Malaysia RUI grant: 1001/PFARMASI/8011003
Weitere Informationen

Publikationsverlauf

received 19. September 2019
revised 18. Februar 2020

accepted 26. Februar 2020

Publikationsdatum:
13. März 2020 (online)

    Lizenzen und Reprints

Abstract

Zingiber montanum rhizomes are traditionally used for the treatment of numerous human ailments. The present study was carried out to investigate the inhibitory activity of the crude extract, chromatographic fractions, and purified compounds from Z. montanum rhizomes on the migration of MDA-MB-231 cells. The effect of the extract on cell migration was investigated by a scratch assay, which showed significant inhibition in a concentration-dependent manner. Vacuum liquid chromatography on silica gel afforded four fractions (Frs. 1 – 4), which were tested on cell migration in the scratch assay. Frs. 1 and 2 showed the most significant inhibition of MDA-MB-231 cell migration. The effect of the most potent fraction (Fr. 2) was further confirmed in a transwell migration assay. The study of Frs. 1 and 2 by gelatin zymography showed significant inhibition of MMP-9 enzyme activity. Chromatographic separation of Frs. 1 and 2 afforded buddledone A (1), zerumbone (2), (2E,9E)-6-methoxy-2,9-humuradien-8-one (3), zerumbone epoxide (4), stigmasterol (5), and daucosterol (6). In a cell viability assay, compounds 1 – 4 inhibited the viability of MDA-MB-231 cells in a concentration-dependent manner. The study of buddledone A (1) and zerumbone epoxide (4) on cell migration revealed that 4 significantly inhibited the migration of MDA-MB-231 cells in both scratch and transwell migration assays. The results of the present study may lead to further molecular studies behind the inhibitory activity of zerumbone epoxide (4) on cell migration and support the traditional use of Z. montanum rhizomes for the treatment of cancer.

Key words

Zingiberaceae - Zingiber montanum rhizomes - inhibitory activity - cancer cell migration - chemical constituents - zerumbone epoxide

Supporting Information

    UV, NMR, and mass spectral data of compounds 1 – 6 are available as Supporting Information. Percentage cell viability of MDA-MB-231 cells treated with the crude extract, Fr. 1, Fr. 2, and compounds 1 – 4, percentage inhibition of MMP-2 activity by Frs. 1 and 2, and the effect of 1 on MDA-MB-231 cell migration are also available as Supporting Information.

  • Supporting Information
 

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