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Planta Med 2019; 85(07): 570-577
DOI: 10.1055/a-0733-7151
Biological and Pharmacological Activity
Original Papers
Georg Thieme Verlag KG Stuttgart · New York

Pro-Fibrogenic and Anti-Inflammatory Potential of a Polyphenol-Enriched Fraction from Annona crassiflora in Skin Repair

Francyelle Borges Rosa de Moura   *
1   Institute of Biomedical Sciences, Federal University of Uberlândia, Uberlandia, MG, Brazil
2   Institute of Biology, State University of Campinas, SP, Brazil
,
Allisson Benatti Justino   *
3   Institute of Biotechnology, Federal University of Uberlandia, Uberlandia, MG, Brazil
,
Bruno Antonio Ferreira
1   Institute of Biomedical Sciences, Federal University of Uberlândia, Uberlandia, MG, Brazil
,
Foued Salmen Espindola
3   Institute of Biotechnology, Federal University of Uberlandia, Uberlandia, MG, Brazil
,
Fernanda de Assis Araújo
1   Institute of Biomedical Sciences, Federal University of Uberlândia, Uberlandia, MG, Brazil
,
Tatiana Carla Tomiosso
1   Institute of Biomedical Sciences, Federal University of Uberlândia, Uberlandia, MG, Brazil
› Author Affiliations
Further Information

Publication History

received 23 May 2018
revised 13 August 2018

accepted 03 September 2018

Publication Date:
20 September 2018 (online)

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Abstract

A polyphenol-enriched fraction from Annona crassiflora fruit peel (PEF-Ac) containing chlorogenic acid, epi-catechin, procyanidins B2 and C1, quercetin-glucoside, kaempferol, and caffeoyl-glucoside was investigated for its anti-inflammatory, pro-angiogenic, and profibrogenic potential in the healing of cutaneous wounds. Four wounds were performed on the back of C57 mice and the lesions were treated with the vehicle (Vaseline and lanolin) and PEF-Ac at concentrations of 2%, 4%, and 6% for 4 and 7 d. Neutrophils and macrophages activities were evaluated indirectly by the activity of myeloperoxidase and N-acetyl-β-D-glycosaminidase, angiogenesis was evaluated by hemoglobin dosing and vessel count in histological sections, and collagen deposition was assessed from histological sections stained with picrosirius red. PEF-Ac demonstrated anti-inflammatory activity, with reduced activities of neutrophil and macrophage in the cutaneous wounds. In addition, there was an increase in the synthesis of types I and III collagen, as well as in the percentage of wound closure, mainly after 4 d of treatment. On the other hand, PEF-Ac did not present an effective pro-angiogenic activity. A. crassiflora fruit peel showed anti-inflammatory and profibrogenic properties, indicating a promising natural source of bioactive molecules for treatment of cutaneous wounds.

Key words

Annona crassiflora - Annonaceae - inflammation - wound healing - collagen - polyphenols

* These authors contributed equally to this manuscript.


 

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