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Thromb Haemost 2006; 96(02): 176-182
DOI: 10.1160/TH06-05-0270
Platelets and Blood Cells
Schattauer GmbH

Leukemic lymphoblasts, a novel expression site of coagulation factor XIII subunit A

Flóra Kiss   *
1   Department of Clinical Biochemistry and Molecular Pathology
,
Zsuzsanna Hevessy   *
1   Department of Clinical Biochemistry and Molecular Pathology
,
Anikó Veszprémi
1   Department of Clinical Biochemistry and Molecular Pathology
,
Éva Katona
2   Clinical Research Center, Thrombosis and Haemostasis Research Group of the Hungarian Academy of Sciences
,
Csongor Kiss
3   Department of Pediatrics
,
György Vereb
4   Department of Biophysics and Cell Biology, Medical and Health Science Center; University of Debrecen, Debrecen, Hungary
,
László Muszbek
2   Clinical Research Center, Thrombosis and Haemostasis Research Group of the Hungarian Academy of Sciences
,
János Kappelmayer
1   Department of Clinical Biochemistry and Molecular Pathology
› Author AffiliationsFinancial support: This work was supported by grants from the University of Debrecen Medical and Health Science Center (Mec 13–2002), the Hungarian National Fund OTKA (T049392 and T043086) and the Hungarian Ministry of Health (ETT 355/2003).
Further Information

Publication History

Received 16 May 2006

Accepted after resubmission 07 July 2006

Publication Date:
28 November 2017 (online)

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Summary

Blood coagulation factor XIII (FXIII) is a protransglutaminase circulating asa tetramer formed by two types of subunits (A2B2). The intracellular dimeric form of FXIII (A2) is present in platelets, megakaryocytes, monocytes and macrophages and has been detected in mono-and megakaryocytic leukemias. The aim of our study was to investigate FXIII-A expression in newly diagnosed B cell acute lymphoblastic leukemia (ALL) samples. We examined 47 de novo ALL cases of B cell origin by triple color labeling with flow cytometry. FXIII-A was detected by a FITC conjugated monoclonal antibody combined with CD34 and CD45 staining. In selected cases FXIII-A was investigated on slides prepared from blasts and visualized with a fluorescent microscope. In addition, blasts were studied by Western blot analysis and FXIII-A was measured by a highly sensitive ELISA method. By flow cytometry 19 samples of the 47 cases were found to be FXIII-A positive. Antigen concentration was 3.11± 1.19 fg/blast, while normal lymphoid precursors and mature lymphocytes from B-CLL did not contain FXIII-A. In the lysate of lymphoblasts that were positive by flow cytometry, a single band (82 kDa) corresponding to FXIII-A was detected on Western blots. Confocal laser scanning microscopic examination revealed the presence of FXIII-A in the cytoplasm of these lymphoblasts. This novel expression site of FXIII-A in leukemic lymphoblasts can be utilized as a diagnostic tool and may also gain functional significance in B-lineage ALL.

Keywords

Coagulation factor XIII - acute lymphoblastic leukemia - flow cytometry

* These authors contributed equally.


 

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