Immobilization of Galphimia glauca Plant Cell Suspensions for the Production of Enhanced Amounts of Galphimine-B

Abstract

We tested the capacity of Galphimia glauca cells to produce galphimine-B (G-B) when under the effects of a two-stage culture system: cell immobilization in Ca²⁺-alginate beads and culture scale-up from shake-flask to two different types of bioreactor (stirred and airlift). In the shake-flask culture, using optimum media for cell growth (first stage) and G-B production (second stage), the G-B yield was similar in both immobilised and free cells. However, while the free cells accumulated G-B within cytoplasmatic compartments, where it could not be recovered without cell disruption, immobilized cells excreted up to 100 % of the G-B produced. Immobilized cells grown in bioreactors running for 14 days with growth medium and an additional 26 days with production medium in batch mode showed a high G-B yield. The stirred bioreactor was the most efficient with a G-B content in the culture medium of 1381 μg.L^-1 at day 24 of culture.

Key words

alginate - anxiolytic activity - excretion - immobilization - Galphimia glauca - galphimine-B - Malpighiaceae - stirred bioreactor

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