Planta Med 2008; 74(1): 94-99
DOI: 10.1055/s-2007-993763
Physiology and Biotechnology
Original Paper
© Georg Thieme Verlag KG Stuttgart · New York

Immobilization of Galphimia glauca Plant Cell Suspensions for the Production of Enhanced Amounts of Galphimine-B

Lidia  Osuna1 , 2 , Elisabeth  Moyano3 , Susana  Mangas1 , Mercè  Bonfill1 , Rosa  M. Cusidó1 , M.  Teresa  Piñol1 , Alejandro  Zamilpa2 , Jaime  Tortoriello2 , Javier  Palazón1
  • 1Facultad de Farmacia, Departamento de Fisiología Vegetal, Universidad de Barcelona, Barcelona, Spain
  • 2Centro de Investigación Biomédica del Sur, Instituto Mexicano del Seguro Social, Morelos, Mexico
  • 3Departament de Ciències Experimentals i de la Salut, Universitat Pompeu Fabra, Barcelona, Spain
Further Information

Publication History

Received: February 28, 2007 Revised: October 5, 2007

Accepted: November 12, 2007

Publication Date:
04 January 2008 (online)

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Abstract

We tested the capacity of Galphimia glauca cells to produce galphimine-B (G-B) when under the effects of a two-stage culture system: cell immobilization in Ca2+-alginate beads and culture scale-up from shake-flask to two different types of bioreactor (stirred and airlift). In the shake-flask culture, using optimum media for cell growth (first stage) and G-B production (second stage), the G-B yield was similar in both immobilised and free cells. However, while the free cells accumulated G-B within cytoplasmatic compartments, where it could not be recovered without cell disruption, immobilized cells excreted up to 100 % of the G-B produced. Immobilized cells grown in bioreactors running for 14 days with growth medium and an additional 26 days with production medium in batch mode showed a high G-B yield. The stirred bioreactor was the most efficient with a G-B content in the culture medium of 1381 μg.L-1 at day 24 of culture.