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Plant Biol (Stuttg) 2006; 8(5): 615-626
DOI: 10.1055/s-2006-924076
Research Paper

Georg Thieme Verlag Stuttgart KG · New York

Localization of Methyl Benzoate Synthesis and Emission in Stephanotis floribunda and Nicotiana suaveolens Flowers

D. Rohrbeck1 , D. Buss1 , U. Effmert1 , B. Piechulla1
  • 1Institute of Biological Sciences, University of Rostock, Albert-Einstein-Straße 3, 18059 Rostock, Germany
Weitere Informationen

Publikationsverlauf

Received: December 23, 2005

Accepted: March 4, 2006

Publikationsdatum:
01. Juni 2006 (online)

   Lizenzen und Reprints

Abstract

The emission of fragrances can qualitatively and quantitatively differ in different parts of flowers. A detailed analysis was initiated to localize the floral tissues and cells which contribute to scent synthesis in Stephanotis floribunda (Asclepiadaceae) and Nicotiana suaveolens (Solanaceae). The emission of scent compounds in these species is primarily found in the lobes of the corollas and little/no emission can be attributed to other floral organs or tissues. The rim and centre of the petal lobes of S. floribunda contribute equally to scent production since the amount of SAMT (salicylic acid carboxyl methyltransferase) and specific SAMT activity compensate each other in the rim region and centre region. In situ immunolocalizations with antibodies against the methyl benzoate and methyl salicylate-synthesizing enzyme indicate that the adaxial epidermis with few subepidermal cell layers of S. floribunda is the site of SAMT accumulation. In N. suaveolens flowers, the petal rim emits twice as much methyl benzoate due to higher total protein concentrations in the rim versus the petal centre; and, both the adaxial and abaxial epidermis house the BSMT (salicylic acid/benzoic acid carboxyl methyltransferase).

Key words

Scent emission - carboxyl methyltransferase - Stephanotis floribunda - Nicotiana suaveolens - cellular localization - methyl benzoate - methyl salicylate.

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B. Piechulla

Institute of Biological Sciences
University of Rostock

Albert-Einstein-Straße 3

18059 Rostock

Germany

eMail: birgit.piechulla@biologie.uni-rostock.de

Editor: E. Pichersky

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