J Reconstr Microsurg 2005; 21(7): 471-476
DOI: 10.1055/s-2005-918902
Copyright © 2005 by Thieme Medical Publishers, Inc., 333 Seventh Avenue, New York, NY 10001, USA.

Functional Recovery of Rat Hind-Limb Allografts

You-Xin Song1 , Keiichi Muramatsu1 , Yoko Kurokawa1 , Ryutaro Kuriyama1 , Sotetsu Sakamoto1 , Kazuo Kaneko1 , Toshihiko Taguchi1
  • 1Department of Orthopedic Surgery, Yamaguchi University School of Medicine, Japan
Further Information

Publication History

Accepted: June 13, 2005

Publication Date:
30 September 2005 (online)

ABSTRACT

Few papers have assessed the long-term functional recovery of animal limb allografts. In this study, the functional recovery of rat limb allografts was serially and quantitatively investigated for a period of 1 year. The donor's hind limb was orthotopically transplanted into the recipient. Fifteen recipients with allografts were treated with FK506. Functional recovery of the grafted limb was assessed serially by cutaneous reaction test, walking track analysis, and electrophysiologic evaluation.

Sensibility improved to a similar extent in both isografts and allografts, and the recovery rate at 1 year was 68 percent, compared to the normal side. Sciatic function index significantly improved to - 70 points after 1 year. The amplitude recorded from the gastrocnemius muscle significantly improved, and the ratio compared to the normal side was 43 percent. Limb isografts and allografts treated with FK506 showed no significant differences in functional recovery. The data can be used as a reference standard for future investigations.

REFERENCES

  • 1 Dubernard J M, Owen E, Herzberg G et al.. Human hand allograft: report on first 6 months.  Lancet. 1999;  353 1315-1320
  • 2 Jones N F. Concerns about human hand transplantation in the 21st century.  J Hand Surg. 2002;  27 771-787
  • 3 Jones J W, Gruber S A, Barker J H, Breidenbach W C. Successful hand transplantation. One-year follow-up. Louisville Hand Transplant Team.  N Engl J Med. 2000;  343 468-473
  • 4 Siemionow M, Ozer K. Advances in composite tissue allograft transplantation as related to the hand and upper extremity.  J Hand Surg. 2002;  27 565-580
  • 5 Doi K. Homotransplantation of limbs in rats: a preliminary report on an experimental study with nonspecific immunosuppressive drugs.  Plast Reconstr Surg. 1979;  64 613-621
  • 6 Arai K, Hotokebuchi T, Miyahara H et al.. Limb allografts in rats immunosuppressed with FK506. I. Reversal of rejection and indefinite survival.  Transplantation. 1989;  48 782-786
  • 7 Benhaim P, Anthony J P, Lin L Y, Mccalmont T H, Mathes S J. A long-term study of allogeneic rat hind limb transplants immunosuppressed with RS-61443.  Transplantation. 1993;  56 911-917
  • 8 Fealy M J, Umansky W S, Bickel K D, Nino J J, Moris R E, Press B H. Efficacy of rapamycin and FK506 in prolonging rat hind limb allograft survival.  Ann Surg. 1994;  219 88-93
  • 9 Muramatsu K, Doi K, Kawai S. Limb allotransplantation in rats: combined immunosuppression by FK-506 and 15-deoxyspergualin.  J Hand Surg. 1999;  24 586-593
  • 10 Maeda N, Ishiguro N, Inoue G, Miura T, Sugimura K. Nerve regeneration in rat composite-tissue allografts.  J Reconstr Microsurg. 1991;  7 297-301
  • 11 Kuroki H, Ikuta Y. Nerve regeneration of vascularized rat limb allograft and functional recovery of long-term graft survivals treated by short course of FK 506 or cyclosporine.  Transplant Proc. 1995;  27 348-350
  • 12 Yeh L S, Gregory C R, Theriault B R, Hou S M, Lecouter R A. A functional model for whole limb transplantation in the rat.  Plast Reconstr Surg. 2000;  105 1704-1711
  • 13 Mathes D W, Randolph M A, Solari M G et al.. Split tolerance to a composite tissue allograft in a swine model.  Transplantation. 2003;  75 25-31
  • 14 Hovius S E, Stevens H P, van Nierop P W, Rating W, van Strik R, van der Meulen J C. Allogeneic transplantation of the radial side of the hand in the rhesus monkey: I. Technical aspects.  Plast Reconstr Surg. 1992;  89 700-709
  • 15 Goto T, Kino T, Hatanaka H et al.. Discovery of FK-506, a novel immunosuppressant isolated from Streptomyces tsukubaesis.  Transplant Proc. 1987;  19 4-8
  • 16 Bain J R, Mackinnon S E, Hudson A R, Falk J A, Hunter D A. The peripheral nerve alograft: a dose-response curve in the rat immunosuppressed with cyclosporin A.  Plast Reconstr Surg. 1988;  82 447-457
  • 17 de Medinaceli L, Freed W J, Wyatt R J. An index of the functional condition of rat sciatic nerve based on measurements made from walking tracks.  Exp Neurol. 1982;  77 634-643
  • 18 Hare G M, Evans P J, Mackinnon S E et al.. Walking track analysis: a long-term assessment of peripheral nerve recovery.  Plast Reconstr Surg. 1992;  89 251-258
  • 19 Thomason P R, Matzke H A. Effects of ischemia on the hind limb of the rat.  Am J Physical Med. 1975;  54 113-131
  • 20 De Koning P, Brakkee J H, Gispen W H. Methods for producing a reproducible crush in the sciatic and tibial nerve of the rat and rapid and precise testing of return of sensory function. Beneficial effects of melanocortins.  Neurol Sci. 1986;  74 237-246
  • 21 Hruska R E, Kennedy S, Silbergeld E K. Quantitative aspects of normal locomotion in rats.  Life Sci. 1979;  25 171-174

Keiichi MuramatsuM.D. 

Department of Orthopedic Surgery, Yamaguchi University School of Medicine

1-1-1 Minami-kogushi, Ube, Yamaguchi 755-8505, Japan