Antioxidant Activity of Saponins Isolated from Ivy: α-Hederin, Hederasaponin-C, Hederacolchiside-E and Hederacolchiside-F

Abstract

The antioxidant activities of α-hederin and hederasaponin-C from Hedera helix, and hederacolchisides-E and -F from Hedera colchica were investigated, in this study. The antioxidant properties of the saponins were evaluated using different antioxidant tests: 1,1-diphenyl-2-picryl-hydrazyl (DPPH·) free radical scavenging, total antioxidant activity, reducing power, superoxide anion radical scavenging, hydrogen peroxide scavenging, and metal chelating activities. α-Hederin, hederasaponin-C, as well as hederacolchisides-E and -F exhibited a strong total antioxidant activity. At the concentration of 75 μg/mL, these saponins showed 94, 86, 88 and 75 % inhibition on lipid peroxidation of linoleic acid emulsion, respectively. These various antioxidant activities were compared with model antioxidants such as α-tocopherol, butylated hydroxyanisole (BHA) and butylated hydroxytoluene (BHT).

References

• 1 Gülçin İ, Oktay M, Kireçci E, Küfreviogõlu Ö İ. Screening of antioxidant and antimicrobial activities of anise (Pimpinella anisum L.) seed extracts.  Food Chem. 2003;  83 371-82
• 2 Süleyman H, Mshvildadze V, Gepdiremen A, Elias R. Acute and chronic anti-inflamatory profile of the ivy plant, Hedera helix, in rats.  Phytomedicine. 2003;  10 370-4
• 3 Swamy S M, Huat B T. Intracellular glutathione depletion and reactive oxygen species generation are important in α-hederin-induced apoptosis of P388 cells.  Mol Cell Biochem. 2003;  245 127-39
• 4 Jeonga H G, Choi C Y. Expression of inducible nitric oxide synthase by α-hederin in macrophages.  Planta Medica. 2002;  68 392-6
• 5 Mba Gachou C, Laget M, Guiraud-Dauriac H, De Meo M, Elias R, Dumenil Mutation G. The protective activity of α-hederine against H₂O₂ genotoxicity in HepG2 cells by alkaline comet assay.  Research. 1999;  445 9-20
• 6 Jeonga H G, Lee S S. Suppressive effects of α-hederin on 2,3,7,8-tetrachlorodibenzo-p-dioxin-mediated murine Cyp1a-1 expression in the mouse hepatoma Hepa-1c1c7 cells.  Cancer Lett. 1999;  138 131-7
• 7 Liu Y P, Liu J. Effect of α-hederin on hepatic detoxifying systems in mice.  Zhongguo Yao Li Xue Bao. 1997;  18 33-6
• 8 Mshvildadze V ., Elias R ., Baghdikian B ., Ollivier E ., Dekanosidze G ., Kemertelidze E ., Balansard G. Qualitative and quantitative HPLC of dry extracts from the leaves of Hedera colchica and H. pastuchowii. 5th International Symposium on the Chemistry of Natural compounds. May 20 - 23, 2003 Tashkent, Uzbekistan;
• 9 Elias R, Diaz-Lanza A M, Vidal-Ollivier E, Balansard G, Faure R, Babadjamin A. Triterpenoid saponins from the leaves Hedera helix .  J Nat Prod. 1991;  54 98-103
• 10 Blois M S. Antioxidant determinations by the use of a stable free radical.  Nature. 1958;  26 1199-200
• 11 Oyaizu M. Studies on product of browning reaction prepared from glucose amine.  Jpn J Nutr. 1986;  44 307-15
• 12 Liu F, Ooi V EC, Chang S T. Free radical scavenging activity of mushroom polysaccharide extracts.  Life Sci. 1997;  60 763 - 71
• 13 Gülçin I, Büyükokurogõlu M E, Oktay M, Küfreviogõlu Ö İ. Antioxidant and analgesic activities of turpentine of Pinus nigra Arn. subsp. pallsiana (Lamb.) Holmboe.  J Ethnopharmcol. 2003;  86 51-8
• 14 Gülçin İ, Beydemir Ş, Alici H A, Elmastaş M, Büyükokurogõlu M E. In vitro antioxidant properties of morphine.  Pharmacol Res. 2003;  49 59-66