The antioxidant activities of α-hederin and hederasaponin-C from Hedera helix, and hederacolchisides-E and -F from Hedera colchica were investigated, in this study. The antioxidant properties of the saponins were evaluated using different antioxidant tests: 1,1-diphenyl-2-picryl-hydrazyl (DPPH·) free radical scavenging, total antioxidant activity, reducing power, superoxide anion radical scavenging, hydrogen peroxide scavenging, and metal chelating activities. α-Hederin, hederasaponin-C, as well as hederacolchisides-E and -F exhibited a strong total antioxidant activity. At the concentration of 75 μg/mL, these saponins showed 94, 86, 88 and 75 % inhibition on lipid peroxidation of linoleic acid emulsion, respectively. These various antioxidant activities were compared with model antioxidants such as α-tocopherol, butylated hydroxyanisole (BHA) and butylated hydroxytoluene (BHT).
References
1
Gülçin İ, Oktay M, Kireçci E, Küfreviogõlu Ö İ.
Screening of antioxidant and antimicrobial activities of anise (Pimpinella anisum L.) seed extracts.
Food Chem.
2003;
83
371-82
2
Süleyman H, Mshvildadze V, Gepdiremen A, Elias R.
Acute and chronic anti-inflamatory profile of the ivy plant, Hedera helix, in rats.
Phytomedicine.
2003;
10
370-4
3
Swamy S M, Huat B T.
Intracellular glutathione depletion and reactive oxygen species generation are important in α-hederin-induced apoptosis of P388 cells.
Mol Cell Biochem.
2003;
245
127-39
5
Mba Gachou C, Laget M, Guiraud-Dauriac H, De Meo M, Elias R, Dumenil Mutation G.
The protective activity of α-hederine against H2O2 genotoxicity in HepG2 cells by alkaline comet assay.
Research.
1999;
445
9-20
6
Jeonga H G, Lee S S.
Suppressive effects of α-hederin on 2,3,7,8-tetrachlorodibenzo-p-dioxin-mediated murine Cyp1a-1 expression in the mouse hepatoma Hepa-1c1c7 cells.
Cancer Lett.
1999;
138
131-7
8 Mshvildadze V ., Elias R ., Baghdikian B ., Ollivier E ., Dekanosidze G ., Kemertelidze E ., Balansard G. Qualitative and quantitative HPLC of dry extracts from the leaves of Hedera colchica and H. pastuchowii. 5th International Symposium on the Chemistry of Natural compounds. May 20 - 23, 2003 Tashkent, Uzbekistan;
9
Elias R, Diaz-Lanza A M, Vidal-Ollivier E, Balansard G, Faure R, Babadjamin A.
Triterpenoid saponins from the leaves Hedera helix
.
J Nat Prod.
1991;
54
98-103
13
Gülçin I, Büyükokurogõlu M E, Oktay M, Küfreviogõlu Ö İ.
Antioxidant and analgesic activities of turpentine of Pinus nigra Arn. subsp. pallsiana (Lamb.) Holmboe.
J Ethnopharmcol.
2003;
86
51-8
