Plant Biol (Stuttg) 2003; 5(5): 513-521
DOI: 10.1055/s-2003-44793
Original Paper

Georg Thieme Verlag Stuttgart · New York

Evidence for the Polyphyly of Haworthia (Asphodelaceae Subfamily Alooideae; Asparagales) Inferred from Nucleotide Sequences of rbcL, matK, ITS1 and Genomic Fingerprinting with ISSR-PCR

J. Treutlein 1 , G. F. Smith 2 , B.-E. van Wyk 3 , M. Wink 1
  • 1Institut für Pharmazie und Molekulare Biotechnologie, Universität Heidelberg, Heidelberg, Germany
  • 2Office of the Chief Director: Research and Scientific Services, National Botanical Institute, Pretoria, Republic of South Africa
  • 3Department of Botany, Rand Afrikaans University, Johannesburg, Republic of South Africa
Further Information

Publication History

Publication Date:
27 November 2003 (online)

Abstract

Four molecular markers have been studied to examine the phylogenetic position of the South African plant genus Haworthia Duval within the succulent Asphodelaceae. Sequence data of the chloroplast genes matK and rbcL were compared to the nuclear markers ITS1 and ISSR (Inter Simple Sequence Repeat) analysis. Both lines of molecular data, chloroplast and nuclear DNA, indicate that Haworthia is polyphyletic, forming two distinct clades. Most taxa previously combined as Haworthia subgenus Haworthia branch off early in the alooid chloroplast trees forming a strongly monophyletic group, whereas subgenus Hexangulares forms a polyphyletic assemblage comprising other alooid genera. The nuclear markers ITS1 and ISSR fingerprinting support the two groups as distinctly different, therefore confirming the division seen in chloroplast DNA. The practical implication is that the generic concept of Haworthia may have to be restricted to H. subgenus Haworthia or alternatively, that the groups of Haworthia be treated as infrageneric taxa within a broadened (Linnaean) concept of Aloe.

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M. Wink

Institut für Pharmazie und Molekulare Biotechnologie
Universität Heidelberg

69120 Heidelberg

Germany

Email: wink@uni-hd.de

Section Editor: F. Salamini