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Exp Clin Endocrinol Diabetes 2003; 111(5): 283-287
DOI: 10.1055/s-2003-41754
Article

J. A. Barth Verlag in Georg Thieme Verlag Stuttgart · New York

A Novel Leptin Receptor Variant with a Conservative Amino Acid Substitution (I359 V) in Body Weight Selected and Unselected Mouse Lines

U. Reichart 1 , U. Renne 2 , B. Aigner 3 , J. Kratzsch 4 , G. A. Brockmann 2
  • 1Ludwig-Boltzmann Institute for Immuno-, Cyto- and Molecular Genetic Research Vienna,Vienna, Austria
  • 2Research Institute for the Biology of Farm Animals, Dummerstorf, Germany
  • 3Institute of Animal Breeding and Genetics, University of Veterinary Medicine Vienna, Vienna, Austria
  • 4Institute of Laboratory Medicine, Clinical Chemistry and Molecular Diagnostics, University of Leipzig, Leipzig, Germany
Further Information

Publication History

Received: July 26, 2002 First decision: October 6, 2002

Accepted: January 21, 2003

Publication Date:
02 September 2003 (online)

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Abstract

Recently published results revealed linkage of obesity traits to a chromosomal region near the leptin receptor gene (Lepr) locus in high body weight selected big and fat DU6i mice. The purpose of this study was the search for variants of the Lepr gene in selected and unselected mouse lines as a candidate for different body composition traits. The complete Lepr cDNA sequence was analysed in DU6i mice. In addition, body weight, abdominal fat weight and serum leptin levels were measured in 42 day old, male mice of the strains DU6i, DUKs, Him : OF1 and DBA/2. Sequence comparison to the published wild type sequence revealed three silent mutations and an amino acid exchange (I359 V) in the C2 domain of the putative leptin binding site in the line DU6i. Compared to the high body weight selected mice also unselected lean control DUKs mice and Him : OF1 mice harbour the amino acid substitution, although they show significantly lower values for body weight, abdominal fat weight and serum leptin levels. Therefore, we assume that the mutation in the C2 domain of Lepr alone might not result in impaired leptin binding or signaling.

Key words

Leptin receptor - obesity - polymorphism

References

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G. A. Brockmann

Research Institute for the Biology of Farm Animals

Wilhelm-Stahl-Allee 2

18196 Dummerstorf

Germany

Phone: + 0493820868703

Fax: + 0 49 38 20 86 87 02

Email: Gudrun.Brockmann@fbn-dummerstorf.de