Effects of Some Vanadyl Coordination Compounds on the In Vitro Insulin Release from Rat Pancreatic Islets

M. T. Conconi; E. De Carlo; S. Vigolo; C. Grandi; G. Bandoli; N. Sicolo; G. Tamagno; P. P. Parnigotto; G. G. Nussdorfer

doi:10.1055/s-2003-41620

Hormone and Metabolic Research, Table of Contents

Horm Metab Res 2003; 35(7): 402-406
DOI: 10.1055/s-2003-41620

Original Basic

Effects of Some Vanadyl Coordination Compounds on the In Vitro Insulin Release from Rat Pancreatic Islets

M. T. Conconi ¹ , E. De Carlo ² , S. Vigolo ¹ , C. Grandi ¹ , G. Bandoli ¹ , N. Sicolo ² , G. Tamagno ² , P. P. Parnigotto ¹ , G. G. Nussdorfer ³

¹Departments of Pharmaceutical Sciences, University of Padua, Padua, Italy
²Departments of Medical and Surgical Sciences, University of Padua, Padua, Italy
³Departments of Human Anatomy and Physiology, University of Padua, Padua, Italy

Abstract

Many lines of evidence indicate that vanadium inorganic salts possess insulin-mimetic and insulinotropic properties. However, they are poorly absorbed, so high oral doses are required to achieve effective plasma concentrations with possible undesirable toxic side-effects ensuing. Various organically-chelated vanadium compounds have been synthesized that are more potent than inorganic vanadium salts in their insulin-like effects due to their greater bioavailability. Unfortunately, little is known about the possible insulin secretagogue action of organic vanadyl coordination compounds. Hence, we investigated the effect of [VO(metformin)₂]·H₂O, [VO(salicylidene-ethylenedimmine)₂] and [VO(pyrrolidine-N-dithiocarbamate)₂](VODTC) on insulin release from isolated rat pancreatic islets, and compared it to that of vanadyl sulfate (VOSO₄). Of the three coordination compounds, only VODTC was found to exert insulin secretagogue action. VODTC, within concentrations ranging from 0.1 to 1.0 mM, enhanced both basal and glucose (11 mM)-stimulated insulin release. The effect involves calcium channels, since it was not appreciable in Ca²⁺-free medium. The stimulating action of VODTC required the presence of the whole metal-chelator complex inasmuch as the chelator DTC alone was ineffective. VOSO₄ was unable to bring about any significant rise in insulin release from isolated islets. Taken together, our findings indicate that VODTC may be considered a potential elective pharmaceutical tool in the therapy of diabetes, especially of type 2, through its concomitant stimulatory effect on insulin secretion and insulin-mimetic action.

Key words

Vanadium - Vanadyl coordination compounds - Pancreatic islets - Insulin - Rat

Full Text

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Prof. G. G. Nussdorfer

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