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Horm Metab Res 2003; 35(3): 137-141
DOI: 10.1055/s-2003-39073
Original Basic

© Georg Thieme Verlag Stuttgart · New York

Effects of Estradiol and Progesterone on Vertebral Collagen, Glycosaminoglycans and Phosphatases in Ovariectomized Adult Rats

V.  Gopala Krishnan1 , J.  Arunakaran1 , P.  Govindarajulu1 , N.  Srinivasan1
  • 1Department of Endocrinology, Dr. Alm Post Graduate Institute of Basic Medical Sciences, University of Madras, Taramani, India
Further Information

Publication History

Received 28 May 2002

Accepted after revision 25 October 2002

Publication Date:
07 May 2003 (online)

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Abstract

Vertebral collagen, glycosaminoglycans (GAGs), tartrate-resistant acid phosphatase (TRAP) and alkaline phosphatase (ALP) were measured in ovariectomized (ovx) adult Wistar rats treated with estradiol (E2) (10 µg/kg BW for 35 days on alternate days, and progesterone (P4) (140 µg/kg BW for 35 days on alternate days) in E2 + P4 treated rats. P4 given alone or in combination with E2 significantly increased the levels of collagen in the vertebral bone. Neither ovx nor E2 treatment altered the concentration of collagen in these rats. Administration of E2 or P4 significantly decreased the concentration of hyaluronic acid (HA), but remaining unaffected when a combination of these steroids was given. In contrast to their effect on HA, E2 and P4 each significantly increased the levels of chondroitin sulfate (CS) in the vertebral bone. The specific activity of ALP was decreased after ovx. E2 and P4 alone or in combination also registered a significant decrease in the activities of ALP and TRAP. The results suggest that E2 and P4 each exert definite effects on vertebral bone turnover in ovariectomized rats.

Key words

Bone - Collagen - Estradiol - Glycosaminoglycans - Ovariectomy - Phosphatases - Progesterone

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Dr. N. Srinivasan

Dr. Alm Post Graduate Institute of Basic Medical Sciences · University of Madras

Taramani · Chennai 600 113 · India

Phone: + 91 (44) 24480784

Fax: + 91 (44) 4926709

Email: n_srini2000@yahoo.com