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Horm Metab Res 2002; 34(1): 21-26
DOI: 10.1055/s-2002-19962
Original Clinical

© Georg Thieme Verlag Stuttgart · New York

Plasma Glucose Regulation and Insulin Secretion in Hypertriglyceridemic Mice

M.  E. C.  Amaral 1 , H.  C. F.  Oliveira 1 , E.  M.  Carneiro 1 , V.  Delghingaro-Augusto 1 , E.  C.  Vieira 1 , J.  A.  Berti 1 , A.  C.  Boschero 1
  • 1Departamento de Fisiologia e Biofísica, Instituto de Biologia, Universidade Estadual de Campinas (UNICAMP), CP Campinas, SP, Brasil
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Publication History

Received 3 April 2001

Accepted after Revision 20 August 2001

Publication Date:
14 August 2002 (online)

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Abstract

In this study, we examined glucose homeostasis and insulin secretion in transgenic mice overexpressing the human apolipoprotein CIII gene (apo CIII tg). These mice have elevated plasma levels of triglycerides, FFA and cholesterol compared to control mice. The body weight, plasma glucose, and insulin levels, glucose disappearance rates, areas under the ipGTT curve for adult (4 - 8 mo. old) and aged (20 - 24 mo. old) apo CIII tg mice and the determination of insulin during the ipGTT were not different from those of control mice. However, an additional elevation of plasma FFA by treatment with heparin for 2 - 4 h impaired the ipGTT responses in apo CIII tg mice compared to saline-treated mice. The glucose disappearance rate in heparin-treated transgenic mice was slightly lower than in heparin-treated controls. Glucose (22.2 mmol/l) stimulated insulin secretion in isolated islets to the same extent in saline-treated control and apo CIII tg mice. In islets from heparin-treated apo CIII tg mice, the insulin secretion at 2.8 and 22.2 mmol glucose/l was lower than in heparin-treated control mice. In conclusion, hypertriglyceridemia per se or a mild elevation in FFA did not affect insulin secretion or insulin resistance in adult or aged apo CIII tg mice. Nonetheless, an additional elevation of FFA induced by heparin in hypertriglyceridemic mice impaired the ipGTT by reducing insulin secretion.

Key words

Glucose Homeostasis - Insulin Secretion - Apolipoprotein CIII - Transgenic Mice

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A. C. Boschero

Departamento de Fisiologia e Biofísica · Instituto de Biologia · Universidade Estadual de Campinas (UNICAMP) · CP 6109 Campinas, SP

13083-970 Brasil

Fax: + 55 (19) 289 3124

Email: boschero@unicamp.br