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J Reconstr Microsurg 2002; 18(2): 121-128
DOI: 10.1055/s-2002-19893
CLINICAL REVIEW

Copyright © 2002 by Thieme Medical Publishers, Inc., 333 Seventh Avenue, New York, NY 10001, USA. Tel.: +1(212) 584-4662

Secondary Transplantation Following a Period of Isografting: Experimental Study in Rats

Mehmet Oguz Yenidünya1 , Yang Dongyoung1 , Sibel Yenidünya2 , Mihaela Hriscu1 , Yasuyoshi Tosa1 , Tetsuji Uemura1 , Yoshiaki Hosaka1 , Toshio Morohoshi2
  • 1Department of Plastic Reconstructive Surgery Showa University School of Medicine Tokyo, Japan
  • 2First Department of Pathology Showa University School of Medicine Tokyo, Japan
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Publication History

Publication Date:
31 January 2002 (online)

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ABSTRACT

The authors describe a study in which groin flaps from 20 Wistar rats were transplanted to another group of 20 Wistar rats and, after various time intervals, the groin flaps were re-transplanted back to the original animals. The goal of the first transfers was to preserve the flap in the second group of animals (isopreservation). During the isopreservation period, the second rat (the preserver) was treated with steroids or FK506 for immunosuppression. Thirty-three free groin flap transfers were performed between 40 rats. If possible, the same flap was transferred twice between two animals, one as an isograft, and other as an autograft following an isopreservation period in 13 pairs of animals. (A second transfer was not possible in seven pairs of animals.) The period for isopreservation varied between 2 days and 1 week. The survival of the flap was observed by visual inspection, laser Doppler flowmeter measurements, and was correlated with serial histopathologic examinations of skin and vessel biopsy specimens, including the anastomosis site. The severity of histopathologic signs that might be related to developing rejection was increased by the preservation time, and was more noticeable after the second transfer. The authors showed that successful secondary transplantation of the rat groin flap with a 1-week follow-up could be achieved, following isopreservation of at least up to 5 days.

KEYWORD

Secondary transplantation - isografting - rat model

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